A phenolic extract from grape by-products and its main hydroxybenzoic acids protect Caco-2 cells against pro-oxidant induced toxicity.
A phenolic extract from grape by-products and its main hydroxybenzoic acids protect Caco-2 cells against pro-oxidant induced toxicity.
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DOI：
10.1016/j.fct.2015.12.005
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发表时间：
2016-02
期刊：
影响因子：
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通讯作者：
Shenli Wang;R. Mateos;L. Goya;Miryam Amigo-Benavent;B. Sarriá;L. Bravo
中科院分区：
文献类型：
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作者：
Shenli Wang;R. Mateos;L. Goya;Miryam Amigo-Benavent;B. Sarriá;L. Bravo
葡萄/葡萄酒行业产生大量的副产品，但对它们的健康促进品质的了解有限。本研究探讨了葡萄酚提取物（GPE）及其酚类化合物没食子酸（GA）和胡萝卜酸（SA）对人肠Caco-2细胞的直接或经叔丁基过氧化氢（t-BOOH）诱导的细胞毒性作用的影响。用0.1-10 μg/mL GPE或0.1-10 μM GA和SA直接处理未产生重大细胞毒性效应，无论是抗氧化防御（谷胱甘肽含量、谷胱甘肽过氧化物酶和还原酶活性）的变化还是蛋白质损伤（羰基）。10 μg/mLGPE、1和10 μM GA和10 μM SA处理20 h后，活性氧（ROS）的产生量明显减少，而10 μg/mLGPE、10 μM GA和10 μM SA处理20 h后，活性氧（ROS）的产生量明显减少。10 μg/mL GPE和1-10 μM GA或10 μM SA显著降低促氧化剂诱导的细胞毒性。1-10 10 μg/mL GPE、1-10 μM GA和10 μM SA均能显著恢复还原型谷胱甘肽，提高谷胱甘肽还原酶和过氧化物酶活性，降低蛋白质氧化损伤。因此，用实际浓度的GPE及其主要羟基苯甲酸处理保护Caco-2细胞免受诱导的氧化应激。
Grape/wine industry produces large amounts of by-products, however knowledge on their health-promoting qualities is limited. This study investigated the effects of a grape phenolic extract (GPE) and its phenolic compounds, gallic acid (GA) and syringic acid (SA) on human intestinal Caco-2 cells, directly or after cytotoxicity induced by tert-butylhydroperoxide (t-BOOH). Direct treatment with 0.1–10 μg/mL GPE, or 0.1–10 μM GA and SA produced no major cytotoxic effect, either changes in antioxidant defences (glutathione content, glutathione peroxidase and reductase activities) or protein damage (carbonyl groups). However, 10 μg/mL GPE, 1 and 10 μM GA and 10 μM SA decreased reactive oxygen species (ROS) production.Pre-treatment with GPE, SA and GA at the same concentrations for 20 h showed that 10 μg/mL GPE and 10 μM GA or SA significantly counteracted ROS increase induced by t-BOOH. 10 μg/mL GPE and 1–10 μM GA or 10 μM of SA significantly reduced pro-oxidant-induced cytotoxicity. 1–10 μg/mL GPE, 1–10 μM GA and 10 μM SA significantly recovered both depleted glutathione and enhanced glutathione reductase and peroxidase activities, and reduced protein oxidative damage. Therefore, treatment with realistic concentrations of GPE and its main hydroxybenzoic acids protected Caco-2 cells against induced oxidative stress.