Aquaporin 1 contributes to chondrocyte apoptosis in a rat model of osteoarthritis.
Aquaporin 1 contributes to chondrocyte apoptosis in a rat model of osteoarthritis.
复制标题
水通道蛋白 1 有助于大鼠骨关节炎模型中的软骨细胞凋亡。
DOI：
10.3892/ijmm.2016.2785
复制
发表时间：
2016-12
影响因子：
5.4
通讯作者：
Cui Y
中科院分区：
文献类型：
--
作者：
Gao H;Gui J;Wang L;Xu Y;Jiang Y;Xiong M;Cui Y
水通道蛋白（Aquaporins，AQP）与多种疾病有关。然而，AQP-1在骨关节炎发病机制中的作用仍不清楚。我们以前发现，AQP-1的表达上调，在骨关节炎软骨和强烈相关的caspase-3的表达和活性。本研究的目的是进一步研究AQP-1表达与骨关节炎大鼠模型软骨细胞凋亡的关系，使用RNA干扰敲低AQP-1。将72只雄性SD大鼠随机分为对照组（n=24）、假手术组（n = 24）和骨关节炎组（n=24）。通过切断前交叉韧带和内侧副韧带并部分切除内侧半月板来诱导骨关节炎。分离培养骨关节炎大鼠软骨细胞。使用shRNA敲低培养的软骨细胞中AQP-1的表达。采用逆转录-定量聚合酶链反应（RT-PCR）检测AQP-1和caspase-3的表达。使用半胱天冬酶-3比色测定法测量半胱天冬酶-3活性。我们的骨关节炎模型中的大鼠表现出严重的软骨损伤。AQP-1的敲低降低了培养的软骨细胞中caspase-3的表达和活性。此外，AQP-1的表达与caspase-3的表达及活性呈正相关。因此，我们的研究结果表明，AQP-1促进caspase-3激活，从而有助于软骨细胞凋亡和骨关节炎的发展。
Aquaporins (AQPs) have been found to be associated with a number of diseases. However, the role of AQP-1 in the pathogenesis of osteoarthritis remains unclear. We previously found that AQP-1 expression was upregulated in osteoarthritic cartilage and strongly correlated with caspase-3 expression and activity. The aim of this study was to further investigate the association of AQP-1 expression with chondrocyte apoptosis in a rat model of osteoarthritis, using RNA interference to knock down AQP-1. For this purspose, 72 male Sprague-Dawley rats were randomly assigned to 3 groups as follows: the control group not treated surgically (n=24), the sham-operated group (n=24), and the osteoarthritis group (n=24). Osteoarthritis was induced by amputating the anterior cruciate ligament and medial collateral ligament and partially excising the medial meniscus. Chondrocytes from the rats with osteoarthritis were isolated and cultured. shRNAs were used to knock down AQP-1 expression in the cultured chondrocytes. The expression of AQP-1 and caspase-3 was determined by reverse transcription-quantitative polymerase chain reaction. Caspase-3 activity was measured using a caspase-3 colorimetric assay. The rats in our model of osteoarthritis exhibited severe cartilage damage. The knockdown of AQP-1 decreased caspase-3 expression and activity in the cultured chondrocytes. In addition, the expression of AQP-1 positively correlated with caspase-3 expression and activity. Thus, the findings of our study, suggest that AQP-1 promotes caspase-3 activation and thereby contributes to chondrocyte apoptosis and to the development of osteoarthritis.
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影响因子：
2.7
作者：
Jablonski, EM;Hughes, FM
通讯作者：
Hughes, FM
DOI：
10.1152/ajprenal.00224.2004
发表时间：
2005-01-01
影响因子：
4.2
作者：
Lee, WK;Bork, U;Thévenod, F
通讯作者：
Thévenod, F
影响因子：
7
作者：
Sandell, L. J.;Xing, X.;Patra, D.
通讯作者：
Patra, D.
影响因子：
8.8
作者：
Lee, Hyun-Gyo;Yang, Jae-Ho
通讯作者：
Yang, Jae-Ho
影响因子：
2.5
作者：
Kliem, H.;Berisha, B.;Schams, D.
通讯作者：
Schams, D.