Volume-dependent glutamate permeation depends on transmembrane ionic strength and extracellular Cl-.
Volume-dependent glutamate permeation depends on transmembrane ionic strength and extracellular Cl-.
复制标题
体积依赖性谷氨酸盐渗透取决于跨膜离子强度和细胞外 Cl-。
DOI：
10.1007/s00232-004-0653-3
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Garber,SS
中科院分区：
文献类型：
--
作者：
Hoffman,MM;Garber,SS
Many mammalian cells regulate their volume by the osmotic movement of water directed by anion and cation flux. Ubiquitous volume-dependent anion currents permit cells to recover volume after swelling in response to a hypotonic environment. This study addressed competition between glutamate (Glu) and Cl−permeation in volume-activated anion currents in order to provide insight into the ionic requirements for volume regulation, volume-dependent anion channel activity and to the architecture of the channel pore. The effect of changing the intracellular molar fraction (MF) of Glu and Cl−on conductance and relative anion permeability was evaluated as a function of the extracellular permeant anion and/or the ionic strength. Relative permeability of Glu to Cl−was determined by measuring reversal potentials under defined ionic conditions. Under conditions with high (150 mM) or low (50 mM) ionic strength solutions on both sides of the membrane, Cl−was always more permeable than Glu. When a transmembrane ionic strength gradient (150 mMextracellular: 50 mMintracellular) was set to drive water into the cell, and in the presence of extracellular Cl−, Glu became up to 16-fold more permeable than Cl−. Replacement of extracellular Cl−with Glu abolished this effect. These results indicate that it is possible for Glu to move into the extracellular environment during volume-regulatory events and they support the emerging role of glutamate as a modulator of anion channel activity.
