ISOLATION AND PRIMARY STRUCTURE OF THE GENE ENCODING FRUCTOSE-1,6-BISPHOSPHATASE FROM SACCHAROMYCES-CEREVISIAE
ISOLATION AND PRIMARY STRUCTURE OF THE GENE ENCODING FRUCTOSE-1,6-BISPHOSPHATASE FROM SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0014-5793(88)80313-2
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发表时间：
1988-08-15
期刊：
影响因子：
3.5
通讯作者：
MECKE, D
中科院分区：
文献类型：
--
作者：
ENTIAN, KD;VOGEL, RF;MECKE, D
克隆了编码酿酒酵母果糖-1，6-二磷酸酶(FBP1)的基因。构建的fbpi：：HIS3缺失突变体不能在乙醇中生长，用克隆的fbp1基因转化后恢复生长。该基因编码一个由347个氨基酸残基组成的蛋白质，相对分子质量为38131。该基因与猪肾皮质和绵羊肝酶的同源性分别为47.7%和46.6%，编码328个氨基酸残基。克隆的启动子大小为318bp，只允许低水平表达该基因。这表明在克隆的DNA片段的上游有一个正激活位点(UAS)。
The gene encodingSaccharomyces cerevisiaefructose‐1,6‐bisphosphatase (FBP1) was isolated. Constructedfbpi::HIS3null mutants were unable to grow with ethanol, and growth was restored after transformation with the clonedfbpgene. The gene codes for a protein of 347 amino acid residues with anMrof 38131. Homology with the pig kidney cortex and the sheep liver enzyme is 47.7% and 46.6%, respectively, within a central core of 328 amino acid residues. The cloned promoter size was 318 bp and allowed only low level expression of the gene. This indicates a positive activation site (UAS) upstream of the cloned DNA fragment.