Spatial and temporal variation in marine bacterioplankton diversity as shown by RFLP fingerprinting of PCR amplified 16S rDNA
Spatial and temporal variation in marine bacterioplankton diversity as shown by RFLP fingerprinting of PCR amplified 16S rDNA
复制标题
DOI：
10.1016/s0168-6496(97)00031-7
复制
发表时间：
1997-09-01
影响因子：
4.2
通讯作者：
PedrosAlio, C
中科院分区：
文献类型：
--
作者：
Acinas, SG;RodriguezValera, F;PedrosAlio, C
我们应用了一种简单的方法，基于对从海水样本中扩增的细菌和古生菌的总16S rDNA的限制性内切酶消化，以比较地中海盆地(从巴塞罗那到巴利阿里群岛)从沿海到近海水域横断面上存在的原核生物多样性。获得了表层和深层样品的叶绿素最大值(DCM)。通过在48小时后重复采样，还研究了短时间内的时间变化。比较样品的消化带模式，以检测存在的细菌或古生菌群的主要变化。对于细菌，自由生活群落和真核生物过滤器保留的群落之间的主要差异被认为是颗粒附着的。相比之下，通过同样的方式收集的古生物并不是作为一个单独的群体出现。另一个主要的变化是与深度有关的，在表层和DCM有显著不同的群落，在400米深处采集了单一样本。另一方面，从大陆平台到近海(最大深度2000m)站的横断面上的变化相对较小。时间(48h)和小尺度空间(几公里)变化最小。
We have applied a simple methodology based on restriction endonuclease digestion of the total bacterial and archaeal 16S rDNA amplified from sea water samples to compare the prokaryotic diversity present along a transect from coastal to offshore waters in the Mediterranean basin (from Barcelona to the Balearic Islands). Samples from the surface and deep-chlorophyll maximum (DCM) were obtained. Temporal Variation during a short span was also investigated by repeating the sampling after 48 h. The patterns of digestion bands from the samples were compared to detect major changes in the bacterial or archaeal groups present. For bacteria the main difference was found between the free-living community and that retained by the eukaryotic filter and assumed to be particle attached. By contrast Archaea collected by the same means did not appear as a separate group. The other main variation was depth-related, with remarkably different communities at the surface, at the DCM, and a single sample taken at 400 m depth. On the other hand, the variation along the transect from the continental platform to an offshore (2000 m maximum depth) station was relatively small. Temporal (48 h) and small scale spatial (a few km) variation was minimal.