NONMOTILITY AND PHAGOCYTIC RESISTANCE OF PSEUDOMONAS-AERUGINOSA ISOLATES FROM CHRONICALLY COLONIZED PATIENTS WITH CYSTIC-FIBROSIS
NONMOTILITY AND PHAGOCYTIC RESISTANCE OF PSEUDOMONAS-AERUGINOSA ISOLATES FROM CHRONICALLY COLONIZED PATIENTS WITH CYSTIC-FIBROSIS
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DOI：
10.1128/iai.62.2.596-605.1994
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发表时间：
1994-02-01
影响因子：
3.1
通讯作者：
SPEERT, DP
中科院分区：
文献类型：
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作者：
MAHENTHIRALINGAM, E;CAMPBELL, ME;SPEERT, DP
虽然铜绿假单胞菌长期定植于大多数老年囊性纤维化（CF）患者，但对其持续存在的细菌特征知之甚少。我们观察到，许多铜绿假单胞菌分离的慢性殖民患者是不动的，并抵抗巨噬细胞的吞噬作用。从20名CF患者中收集铜绿假单胞菌分离株，用于多达10个Sears。从早期殖民的菌株是高度能动的，并表示鞭毛蛋白和菌毛蛋白。然而，许多来自慢性定植患者的分离株缺乏鞭毛蛋白表达，并且不运动;共检查了1，030株铜绿假单胞菌CF分离株，其中39%不运动。此外，从几个CF患者中回收的连续分离株在多达10个齿轮中始终不动。缺乏运动性在环境分离株（1.4%）和其他临床分离株（3.7%）的铜绿假单胞菌中很少见。通过引入质粒pPT 212上携带的rpoN基因座的额外拷贝，实现了非运动性铜绿假单胞菌分离株中运动性的部分互补，表明交替的σ因子RpoN可能参与CF感染期间毒力因子的协调调节。我们推测，非运动表型可能提供铜绿假单胞菌在慢性CF感染的生存优势，使其能够抵抗吞噬和保存能量。
Although Pseudomonas aeruginosa chronically colonizes most older patients with cystic fibrosis (CF), bacterial features responsible for its persistence are understood poorly. We observed that many P. aeruginosa isolates from chronically colonized patients were nonmotile and resistant to phagocytosis by macrophages. P. aeruginosa isolates were collected from 20 CF patients for up to 10 Sears. Isolates from early colonization were highly motile and expressed both flagellin and pilin. However, many isolates from chronically colonized patients lacked flagellin expression and were nonmotile; a total of 1,030 P. aeruginosa CF isolates were examined, of which 39% were nonmotile. Moreover, sequential isolates recovered from several of the CF patients were consistently nonmotile for up to 10 gears. Lack of motility was rare among environmental isolates (1.4%) and other clinical isolates (3.7%) of P. aeruginosa examined. Partial complementation of motility in nonmotile P. aeruginosa isolates was achieved by introduction of extra copies of the rpoN locus carried on plasmid pPT212, indicating that the alternate sigma factor, RpoN, may be involved in the coordinate regulation of virulence factors during CF infection. We hypothesize that the nonmotile phenotype may provide P. aeruginosa a survival advantage in chronic CF infection by enabling it to resist phagocytosis and conserve energy.