CD4-independent binding of SIV gp120 to rhesus CCR5
CD4-independent binding of SIV gp120 to rhesus CCR5
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DOI：
10.1126/science.278.5342.1470
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发表时间：
1997-11-21
期刊：
影响因子：
56.9
通讯作者：
Gerard, NP
中科院分区：
文献类型：
--
作者：
Martin, KA;Wyatt, R;Gerard, NP
CCR5和CD4是免疫缺陷病毒进入靶细胞的辅助受体，来自人类免疫缺陷病毒株HIV-1（YU2）的gp120包膜糖蛋白仅在CD4存在时结合人CCR5（CCR5(hu)）或恒河猴CCR5（CCR5(rh)）。来自猴免疫缺陷病毒株SIVmac239的gp120在没有CD4的情况下结合CCR5(rh)，但CCR5（hu）仍然依赖CD4。SIVmac239 gp120的cd4非依赖性结合依赖于CCR5（rh）氨基末端的单一氨基酸Asp（13）。因此，免疫缺陷病毒包膜糖蛋白上的CCR5结合片段可以通过与CD4相互作用或与CCR5氨基末端直接相互作用产生。这些结果可能对慢病毒中受体使用的进化以及有效干预措施的开发具有启示意义。
CCR5 and CD4 are coreceptors for immunodeficiency virus entry into target cells, The gp120 envelope glycoprotein from human immunodeficiency virus strain HIV-1(YU2) bound human CCR5 (CCR5(hu)) or rhesus macaque CCR5 (CCR5(rh)) only in the presence of CD4. The gp120 from simian immunodeficiency virus strain SIVmac239 bound CCR5(rh) without CD4, but CCR5(hu) remained CD4-dependent. The CD4-independent binding of SIVmac239 gp120 depended on a single amino acid, Asp(13), in the CCR5(rh) amino-terminus. Thus, CCR5-binding moieties on the immunodeficiency virus envelope glycoprotein can be generated by interaction with CD4 or by direct interaction with the CCR5 amino-terminus. These results may have implications for the evolution of receptor use among lentiviruses as well as utility in the development of effective intervention.