Fracture healing in protease-activated receptor-2 deficient mice
Fracture healing in protease-activated receptor-2 deficient mice
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DOI：
10.1002/jor.22071
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发表时间：
2012-08-01
影响因子：
2.8
通讯作者：
Schoenecker, Jonathan G.
中科院分区：
文献类型：
--
作者：
O'Neill, Kevin R.;Stutz, Christopher M.;Schoenecker, Jonathan G.
蛋白酶激活受体 2 (PAR-2) 在细胞外蛋白酶和细胞炎症反应启动之间提供了重要的联系。 PAR-2 对骨折愈合的影响尚不清楚。本研究通过评估野生型 (PAR-2+/+) 和敲除型 (PAR-2-/-) 小鼠之间的差异，研究 PAR-2 缺失对骨折愈合的体内影响。髓内固定后，对 34 只 PAR-2+/+ 和 28 只 PAR-2-/- 小鼠进行单侧股骨中段骨折。在骨折后 1、2 和 4 周 (wpf) 进行组织学评估，并在骨折后 7 周和 10 周进行放射线照相（普通放射线照片、微型计算机断层扫描 (mu CT)）和生物力学（扭转测试）评估。对骨折和未骨折的对侧股骨标本进行了评估。根据 mu CT 图像确定极惯性矩 (pMOI)、组织矿物质密度 (TMD)、骨体积分数 (BV/TV)，并根据平片确定骨痂直径。通过 mu CT 评估，在 7 周和 10 周时，PAR-2-/- 和 PAR-2+/+ 小鼠之间的愈伤组织形态存在统计学上的显着差异。然而，基因型之间没有发现显着的组织学、平片或生物力学差异。通过 mu CT 评估发现，PAR-2 的缺失会改变愈伤组织形态，但并未发现对年轻小鼠的骨折愈合产生其他影响。 (C) 2012 年骨科研究学会。由 Wiley periodicals, Inc. 出版。J Orthop Res 30:12711276, 2012
Protease-activated receptor-2 (PAR-2) provides an important link between extracellular proteases and the cellular initiation of inflammatory responses. The effect of PAR-2 on fracture healing is unknown. This study investigates the in vivo effect of PAR-2 deletion on fracture healing by assessing differences between wild-type (PAR-2+/+) and knock-out (PAR-2-/-) mice. Unilateral mid-shaft femur fractures were created in 34 PAR-2+/+ and 28 PAR-2-/- mice after intramedullary fixation. Histologic assessments were made at 1, 2, and 4 weeks post-fracture (wpf), and radiographic (plain radiographs, micro-computed tomography (mu CT)) and biomechanical (torsion testing) assessments were made at 7 and 10 wpf. Both the fractured and un-fractured contralateral femur specimens were evaluated. Polar moment of inertia (pMOI), tissue mineral density (TMD), bone volume fraction (BV/TV) were determined from mu CT images, and callus diameter was determined from plain radiographs. Statistically significant differences in callus morphology as assessed by mu CT were found between PAR-2-/- and PAR-2+/+ mice at both 7 and 10 wpf. However, no significant histologic, plain radiographic, or biomechanical differences were found between the genotypes. The loss of PAR-2 was found to alter callus morphology as assessed by mu CT but was not found to otherwise effect fracture healing in young mice. (C) 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:12711276, 2012