Interaction between biofilm development, structure and detachment in rotating annular reactors
Interaction between biofilm development, structure and detachment in rotating annular reactors
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DOI：
10.1007/s00449-008-0212-x
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发表时间：
2008-10-01
影响因子：
3.8
通讯作者：
Neu, Thomas R.
中科院分区：
文献类型：
--
作者：
Garny, Kerstin;Horn, Harald;Neu, Thomas R.
在生物技术中，生物膜和悬浮物的组成对系统性能或产品质量至关重要。因此，了解生物膜动力学对任何工艺优化都很重要。本研究的目的是研究不同水动力条件和不同葡萄糖负荷下生物膜的发育和脱离。共聚焦激光扫描显微镜是一种快速的方法，可以提供生物膜和离体生物量中细菌和细胞外聚合物（EPS）的结构、分布和体积比的信息。结果表明，生物膜的密度和EPS体积在很大程度上受水动力条件的影响。此外，实验还表明，底物负荷对EPS：细菌的比例和分布有很大影响。最后，生物膜的结构和发育特征反映在离体生物量的组成和数量上。
In biotechnology, composition of biofilms and suspended bioaggregates can be crucial for system performance or product quality. Consequently, understanding biofilm dynamics is important for any process optimisation. The aim of this study was to investigate biofilm development and detachment under different hydrodynamic conditions and varying glucose load. Confocal laser scanning microscopy proved to be a fast method providing information about structure, distribution and volume ratio of bacteria and extra cellular polymers (EPS) within biofilms and detached biomass. As a result, it could be shown that biofilm structure, in terms of density and EPS volume, was largely influenced by hydrodynamic conditions. Furthermore, it was demonstrated that the EPS:bacteria ratio and distribution was largely influenced by substrate load. Finally, the characteristics in biofilm structure and development were reflected in the composition and quantity of the detached biomass.