Reassessing the role of phosphocaveolin-1 in cell adhesion and migration
Reassessing the role of phosphocaveolin-1 in cell adhesion and migration
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DOI：
10.1111/j.1600-0854.2007.00653.x
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发表时间：
2007-12-01
期刊：
影响因子：
4.5
通讯作者：
Parat, Marie-Odile
中科院分区：
文献类型：
--
作者：
Hill, Michelle M.;Scherbakov, Nadja;Parat, Marie-Odile
虽然在发现小窝蛋白-1的早期就发现了酪氨酸14的磷酸化，但这种修饰的功能意义仍然难以捉摸。最近的证据表明，窖蛋白-1酪氨酸14磷酸化在细胞粘附和迁移中的作用。这些结果是基于各种工具，包括广泛使用的小鼠单克隆抗磷酸小窝蛋白-1抗体，其标记，在培养的细胞中，定位于或接近粘着斑的蛋白质。我们在这里报告的结果，从三个独立的实验室，表明该抗体识别phosphocaveolin-1在其他蛋白质中的免疫印迹分析，并与该抗体在免疫染色实验中获得的信号部分是由于桩蛋白的标记。需要解释发表的数据，记住使用该抗体获得的磷酸小窝蛋白-1细胞定位的图像是无效的。我们重新评估目前的知识小窝蛋白-1在细胞粘附和迁移中的作用，鉴于这一新的信息。
Although phosphorylation on tyrosine 14 was identified early in the discovery of caveolin-1, the functional significance of this modification still remains elusive. Recent evidence points to a role of caveolin-1 tyrosine 14 phosphorylation in cell adhesion and migration. These results are based on a variety of tools, including a widely used mouse monoclonal anti-phosphocaveolin-1 antibody, which labels, in cultured cells, a protein localized at or near focal adhesions. We here report results from three independent laboratories, showing that this antibody recognizes phosphocaveolin-1 amongst other proteins in immunoblot analyses and that the signal obtained with this antibody in immunostaining experiments is in part due to labeling of paxillin. Published data need to be interpreted keeping in mind that images of phosphocaveolin-1 cellular localization obtained using this antibody are not valid. We re-evaluate the current knowledge about the role of caveolin-1 in cell adhesion and migration in view of this new information.