Comparison of fluorescent intercalating dyes for quantitative loop-mediated isothermal amplification (qLAMP)
Comparison of fluorescent intercalating dyes for quantitative loop-mediated isothermal amplification (qLAMP)
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DOI：
10.2144/000114432
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发表时间：
2016-07-01
期刊：
影响因子：
2.7
通讯作者：
Filipenko, Maksim L.
中科院分区：
文献类型：
--
作者：
Oscorbin, Igor P.;Belousova, Ekaterina A.;Filipenko, Maksim L.
实时或定量环介导等温扩增（qLAMP）是一种很有前途的技术，用于准确检测生物体和环境中的病原体。本文比较研究了SYTO-9、SYTO-13、SYTO-82、SYBR绿色I、SYBR Gold、EvaGreen六种荧光嵌入染料在三种不同的qLAMP模型体系中的性能。具有最佳结果的SYTO-9和SYTO-82用于另外的酶和模板滴定研究。SYTO-82表现出阈值时间（Tt）和信噪比（SNR）的最佳组合。
Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes-SYTO-9, SYTO-13, SYTO-82, SYBR Green I, SYBR Gold, EvaGreen-in three different qLAMP model systems. SYTO-9 and SYTO-82, which had the best results, were used for additional enzyme and template titration studies. SYTO-82 demonstrated the best combination of time-to-threshold (Tt) and signal-to-noise ratio (SNR).