Development of microarray-based diagnostics of voles and shrews for use in biodiversity monitoring studies, and evaluation of mitochondrial cytochrome oxidase I vs. cytochrome b as genetic markers
Development of microarray-based diagnostics of voles and shrews for use in biodiversity monitoring studies, and evaluation of mitochondrial cytochrome oxidase I vs. cytochrome b as genetic markers
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DOI：
10.1111/j.1365-294x.2004.02126.x
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发表时间：
2004-05-01
影响因子：
4.9
通讯作者：
Frey, JE
中科院分区：
文献类型：
--
作者：
Pfunder, M;Holzgang, O;Frey, JE
分子生物学方法被广泛应用于哺乳动物的物种鉴定。特别是，线粒体细胞色素B基因序列已被证明有助于这一目的。微阵列技术现在可以为生物多样性监测开辟新的前景。有了微阵列，成千上万的基因特征可以在一个叫做“芯片”的显微镜载玻片上进行测试。例如，“哺乳动物芯片”可以包括冗余的诊断标记，以明确识别所有欧洲哺乳动物物种。更广泛的使用，因此经济上更相关，可以是一个'生物多样性芯片'，包含诊断功能，以区分关键物种的分类群的细菌，地衣，软体动物，昆虫，真菌，哺乳动物等任何混合门芯片的一个重要先决条件是标准化的方法。细胞色素氧化酶I是最有希望作为所有真核生物通用标记的基因之一。我们表明，细胞色素氧化酶I是足够的不同物种的田鼠和鼩的聚类分析的歧视。基于这些结果，我们提出了一个诊断芯片使用细胞色素氧化酶I序列的识别Sorex（Soricidae，食虫目）和4种田鼠（Arvicolinae，啮齿目）。我们的结论是，细胞色素氧化酶I可以用作细胞色素B在混合门芯片的替代标记，或两个基因可以组合使用，以提高reddish，从而鲁棒性的特定芯片，包括小型哺乳动物。
Molecular methods are widely used for species identification of mammals. In particular, the mitochondrial cytochrome b gene sequence has proven helpful for this purpose. Microarray technology can now open up new perspectives for biodiversity monitoring. With microarrays, many thousands of genetically based characteristics can be tested on one microscopic glass slide called a 'chip'. A 'Mammalia-Chip', for example, could include redundant diagnostic markers to unambiguously identify all European mammal species. Of broader use, and therefore economically more relevant, could be a 'Biodiversity-Chip', containing diagnostic features to distinguish key species in the taxa of bacteria, lichen, molluscs, insects, fungi, mammals, etc. An important prerequisite for any mixed-phyla chip is a standardization of methods. One of the most promising genes as a universal marker for all eukaryotes is cytochrome oxidase I. We show that cytochrome oxidase I is adequate for the discrimination of different species of voles and shrews with cluster analysis. Based on these results we present a diagnostic microarray-chip using cytochrome oxidase I sequences for the identification of three species of Sorex (Soricidae, Insectivora) and four species of Microtus (Arvicolinae, Rodentia). We conclude that cytochrome oxidase I can be used as an alternative marker to cytochrome b in a mixed-phyla chip, or both genes can be used in combination to enhance redundance and thus robustness of a specific chip including small mammals.