Angiotensin II and PDGF-BB stimulate β1-integrin-mediated adhesion and spreading in human VSMCs
Angiotensin II and PDGF-BB stimulate β1-integrin-mediated adhesion and spreading in human VSMCs
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DOI：
10.1161/01.hyp.35.1.255
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发表时间：
2000-01-01
期刊：
影响因子：
8.3
通讯作者：
Graf, K
中科院分区：
文献类型：
--
作者：
Kappert, K;Schmidt, G;Graf, K
β（1）-整合素在人平滑肌细胞的粘附和伸展中起重要作用。在本研究中，我们研究了血管紧张素II和血小板衍生生长因子（PDGF）-BB对从髂动脉获得的人平滑肌细胞的β（1）-整合素依赖性功能的影响。用PDGF-BB（20 ng/mL）和血管紧张素II（1 μ mol/L）处理这些细胞长达48小时，通过流式细胞术和逆转录聚合酶链反应分析，β（1）-整合素表达没有改变。β 1整合素主要介导人平滑肌细胞与I型胶原的粘附（79.7 ± 4.4%，P
beta(1)-Integrins play an important role for adhesion and spreading of human smooth muscle cells. In the present study we examined the influence of angiotensin II and platelet-derived growth factor (PDGF)-BB on beta(1)-integrin-dependent functions of human smooth muscle cells obtained from iliac arteries. Treatment of these cells with PDGF-BB (20 ng/mL) and Angiotensin II (1 mu mol/L) did not change beta(1)-integrin expression up to 48 hours as analyzed by flow cytometry and reverse transcription polymerase chain reaction. beta(1)-integrins predominantly mediated adhesion of human smooth muscle cells to collagen I (79.7+/-4.4%, P