Diverse supramolecular structures formed by self-assembling proteins of the Bacillus subtilis spore coat.
Diverse supramolecular structures formed by self-assembling proteins of the Bacillus subtilis spore coat.
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DOI：
10.1111/mmi.13030
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发表时间：
2015-07
影响因子：
3.6
通讯作者：
Bullough PA
中科院分区：
文献类型：
--
作者：
Jiang S;Wan Q;Krajcikova D;Tang J;Tzokov SB;Barak I;Bullough PA
细菌孢子（内生孢子），如病原体艰难梭菌和炭疽杆菌B的那些，是独特的稳定细胞形式，对恶劣的环境损害具有高度抗性。B acillus subtilis是研究得最好的产孢菌，我们已经用它来解决孢子外壳如何由多个组分组装成坚固的保护性超结构的问题。在大肠杆菌中表达的B . subtilis外壳蛋白（CotY、CotE、CotV和CotW）可以通过自组装过程在细胞内排列成高度稳定的宏观结构。使用电子显微镜，我们证明了这些蛋白质产生有序的一维纤维，二维片和三维堆叠的能力。在一种情况下（CotY），高度有序有利于强的、协同的细胞内二硫键交联。这种组装可以形成精致的适应性构建块，用于所有孢子形成者的高阶组装。这些物理上坚固的阵列单元也可以在纳米生物技术过程中有新的应用。
Bacterial spores (endospores), such as those of the pathogens C lostridium difficile and B acillus anthracis, are uniquely stable cell forms, highly resistant to harsh environmental insults. B acillus subtilis is the best studied spore‐former and we have used it to address the question of how the spore coat is assembled from multiple components to form a robust, protective superstructure. B . subtilis coat proteins (CotY, CotE, CotV and CotW) expressed in E scherichia coli can arrange intracellularly into highly stable macro‐structures through processes of self‐assembly. Using electron microscopy, we demonstrate the capacity of these proteins to generate ordered one‐dimensional fibres, two‐dimensional sheets and three‐dimensional stacks. In one case (CotY), the high degree of order favours strong, cooperative intracellular disulfide cross‐linking. Assemblies of this kind could form exquisitely adapted building blocks for higher‐order assembly across all spore‐formers. These physically robust arrayed units could also have novel applications in nano‐biotechnology processes.