A novel WD-repeat protein, WDR26, inhibits apoptosis of cardiomyocytes induced by oxidative stress
A novel WD-repeat protein, WDR26, inhibits apoptosis of cardiomyocytes induced by oxidative stress
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DOI：
10.3109/10715762.2012.678840
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发表时间：
2012-06-01
影响因子：
3.3
通讯作者：
Xiao, Xianzhong
中科院分区：
文献类型：
--
作者：
Feng, Yansheng;Zhang, Chi;Xiao, Xianzhong
WD40重复蛋白具有多种功能，如信号转导、转录调控、细胞周期调控、自噬和凋亡等。WDR26是WD40重复蛋白中的一个新蛋白，在心肌细胞缺血预处理（IPC）过程中上调表达，但其在氧化应激诱导的心肌细胞凋亡及其亚细胞定位中的作用尚不清楚。因此，我们研究了WDR26在大鼠心肌缺血-再灌注损伤模型和H2O2刺激的H9c2细胞中的亚细胞定位和表达。结果表明，WDR26可定位于线粒体，受缺血再灌注损伤和H2O2诱导。然后检测H2O2对H9c2细胞WDR26表达的影响。结果表明，表达WDR26可抑制H2O2诱导的细胞凋亡。此外，我们证明WDR26抑制线粒体释放细胞色素c。这些发现表明WDR26保护心肌细胞免受氧化应激。
WD40 repeat proteins have a variety of functions, such as signal transduction, transcription regulation, cell cycle control, autophagy and apoptosis. WDR26 is a novel protein of WD40 repeat proteins and up-regulated during myocardial cells ischemic preconditioning (IPC) but its role in myocardial cell apoptosis induced by oxidative stress and its subcellular localisation are not clear. So we investigated the subcellular localisation of WDR26 and WDR26 expression in rat myocardial ischaemia-reperfusion injury model and H9c2 cells stimulated by H2O2. The results showed that WDR26 can be located at mitochondria and induced by ischaemia-reperfusion injury and H2O2. Then we examined the effects induced by H2O2 in H9c2 cells WDR26 expression. The results showed that WDR26 expression can inhibit apoptosis induced by H2O2. Further, we demonstrated that WDR26 inhibit cytochrome c release from mitochondria. These founding indicate that WDR26 protects myocardial cells against oxidative stress.