Cellular micromasonry: biofabrication with single cell precision
Cellular micromasonry: biofabrication with single cell precision
复制标题
细胞微砌体：单细胞精度的生物制造
DOI：
10.1039/d2sm01013e
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发表时间：
2022
期刊：
影响因子：
3.4
通讯作者：
Angelini, Thomas E.
中科院分区：
文献类型：
--
作者：
Ellison, S. Tori;Duraivel, Senthilkumar;Subramaniam, Vignesh;Hugosson, Fredrik;Yu, Bo;Lebowitz, Joseph J.;Khoshbouei, Habibeh;Lele, Tanmay P.;Martindale, Mark Q.;Angelini, Thomas E.
在许多组织中，细胞类型在单细胞长度尺度上变化，产生对生理功能至关重要的详细异质性。为了了解组织功能和详细结构之间的关系，并最终设计出结构和生理上准确的组织，我们需要能够组装具有活组织中发现的细节水平的3D细胞结构。在这里，我们介绍了一种方法的3D细胞组装具有比单细胞规模的精度水平。通过这种方法，我们创建了详细的细胞模式，证明了细胞类型可以在单细胞规模上变化，并在组装后显示功能。
In many tissues, cell type varies over single-cell length-scales, creating detailed heterogeneities fundamental to physiological function. To gain understanding of the relationship between tissue function and detailed structure, and eventually to engineer structurally and physiologically accurate tissues, we need the ability to assemble 3D cellular structures having the level of detail found in living tissue. Here we introduce a method of 3D cell assembly having a level of precision finer than the single-cell scale. With this method we create detailed cellular patterns, demonstrating that cell type can be varied over the single-cell scale and showing function after their assembly.