Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes.
Gtsf1/Cue110, a gene encoding a protein with two copies of a CHHC Zn-finger motif, is involved in spermatogenesis and retrotransposon suppression in murine testes.
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DOI：
10.1016/j.ydbio.2009.09.003
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发表时间：
2009-11
影响因子：
2.7
通讯作者：
Takuji Yoshimura;Shuichi Toyoda;Satomi Kuramochi-Miyagawa;Tatsushi Miyazaki;S. Miyazaki;F. Tashiro;
中科院分区：
文献类型：
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作者：
Takuji Yoshimura;Shuichi Toyoda;Satomi Kuramochi-Miyagawa;Tatsushi Miyazaki;S. Miyazaki;F. Tashiro;
我们最近报道，Gtsf1/Cue110 基因是进化上保守的 UPF0224 家族的成员，主要在雄性生殖细胞中表达，并且 GTSF1/CUE110 蛋白定位于成年睾丸中这些细胞的细胞质。在这里，为了分析 Gtsf1/Cue110 基因在精子发生中的作用，我们通过基因打靶产生了 Gtsf1/Cue110 缺失小鼠。 Gtsf1/Cue110缺失的小鼠生长正常并且看起来健康；然而，雄性在出生后第 14 天后由于生殖细胞大量凋亡而无法生育。相反，无效的雌性则具有生育能力。详细分析表明，Gtsf1/Cue110 缺失的雄性减数分裂细胞在合子期之前停止减数分裂进程。因此，Gtsf1/Cue110 基因对于减数分裂早期之后的精子发生至关重要。此外，Gtsf1/Cue110基因的缺失导致长散布核苷酸元件（Line-1）和脑池内A粒子（IAP）反转录转座子的转录增加，并伴随着它们的启动子区域的去甲基化。这些观察结果表明 Gtsf1/Cue110 是精子发生所必需的，并参与男性生殖细胞中的逆转录转座子抑制。
We recently reported that the Gtsf1/Cue110 gene, a member of the evolutionarily conserved UPF0224 family, is expressed predominantly in male germ cells, and that the GTSF1/CUE110 protein is localized to the cytoplasm of these cells in the adult testis. Here, to analyze the roles of the Gtsf1/Cue110 gene in spermatogenesis, we produced Gtsf1/Cue110-null mice by gene targeting. The Gtsf1/Cue110-null mice grew normally and appeared healthy; however, the males were sterile due to massive apoptotic death of their germ cells after postnatal day 14. In contrast, the null females were fertile. Detailed analyses revealed that the Gtsf1/Cue110-null male meiocytes ceased meiotic progression before the zygotene stage. Thus, the Gtsf1/Cue110 gene is essential for spermatogenesis beyond the early meiotic phase. Furthermore, the loss of the Gtsf1/Cue110 gene caused increased transcription of the long interspersed nucleotide element (Line-1) and the intracisternal A-particle (IAP) retrotransposons, accompanied by demethylation of their promoter regions. These observations indicate that Gtsf1/Cue110 is required for spermatogenesis and involved in retrotransposon suppression in male germ cells.