Effect of Plumbago zeylanica extract and certain curing agents on multidrug resistant bacteria of clinical origin
Effect of Plumbago zeylanica extract and certain curing agents on multidrug resistant bacteria of clinical origin
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DOI：
10.1023/a:1008991724288
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发表时间：
2000-01-01
影响因子：
4.1
通讯作者：
Ahmad, I
中科院分区：
文献类型：
--
作者：
Beg, AZ;Ahmad, I
对白花丹（根）的酒精提取物进行了针对细菌（副伤寒沙门氏菌、金黄色葡萄球菌、大肠杆菌、志贺氏菌和携带R质粒的标准菌株大肠杆菌x（+））的多重耐药临床分离株的测试。该提取物对所有测试细菌都表现出很强的抗菌活性，而不管它们的抗生素耐药性。粗提物的植物化学分析表明，黄酮类化合物，皂苷和萘醌的存在。对大肠杆菌R质粒消除方法进行了比较评价。coli x（+）（pUK 651），制备了DNA嵌入染料（吖啶橙子和溴化乙锭）和DNA促旋酶拮抗剂（perfoxacin）。这些药物在各自的亚MIC浓度下均能有效地治疗R质粒。培氟沙星对质粒的清除率最高（88%），其次是溴化乙锭（36%）、吖啶橙子（14%）和锡兰假单胞菌醇提物（14%）。质粒pUK651从E.通过测定固化衍生物培养物中抗性标记物的损失来确认大肠杆菌X（+）。
Alcoholic extract of Plumbago zeylanica (root) was tested against multidrug-resistant clinical isolates of bacteria (Salmonella paratyphi, Staphylococcus aureus, Escherichia coli, Shigella dysenteriae and a R-plasmid-harbouring standard strain, E.coli x(+)). The extract exhibited strong antibacterial activity against all test bacteria irrespective of their antibiotic resistance behaviour. Phytochemical analysis of crude extract revealed the presence of flavonoids, saponins and naphthoquinone. A comparative evaluation of R-plasmid elimination from E. coli x(+) (pUK 651) by the plant extract, DNA intercalating dyes (acridine orange and ethidium bromide) and a DNA gyrase antagonizing drug (perfoxacin) were made. All these agents could cure R-plasmid effectively at their respective sub-MIC concentrations. Maximum plasmid curing was observed by pefloxacin (88%), followed by ethidium bromide (36%), acridine orange (14%) and alcoholic extract of P. zeylanica (14%). Curing of plasmid pUK651 from E. coli x(+) was confirmed by determining the loss of resistance markers in the cured derivative culture.