Effects of estradiol and progesterone on immunoreactive forms of hypothalamic luteinizing hormone-releasing hormone.
Effects of estradiol and progesterone on immunoreactive forms of hypothalamic luteinizing hormone-releasing hormone.
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雌二醇和黄体酮对下丘脑黄体生成素释放激素免疫反应形式的影响。
DOI：
10.1159/000124505
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发表时间：
1986
影响因子：
4.1
通讯作者：
C. Kordon
中科院分区：
文献类型：
--
作者：
S. Drouva;J. Gautron;E. Pattou;E. Laplante;C. Kordon
为了探讨卵巢激素对下丘脑促黄体生成素释放激素（LHRH）神经元作用的机制，在下丘脑亚细胞级分中用不同特异性的抗体免疫测定十肽的分子量（MW 1，800道尔顿），在用含有0.02%叠氮化钠的0.2N乙酸平衡的Biogel P4柱上进行分子筛过滤后。本研究在去卵巢（OVX）、去卵巢雌二醇植入（OVX + E_2）和去卵巢雌二醇+孕酮治疗（OVX + E_2 + P）大鼠中进行。在黄体生成素（LH）昼夜节律峰之前或期间处死动物。在OVX + E2-植入动物中，从突触体组分中恢复的LHRH样免疫反应性的量略有增加，但在OVX + E2 + P-治疗的大鼠中非常明显地增加。与此相反，当与其他组相比时，在OVX + E2 + P处理的大鼠中，从高速上清液中回收的高分子形式显著降低。在E2 + P诱导的LH最大释放时，下丘脑LHRH明显减少，而大分子形式明显增加。这些数据表明，卵巢类固醇激素不仅影响下丘脑LHRH的释放，而且还影响LHRH前体形式的加工。
In order to investigate mechanisms underlying the ovarian steroid action on hypothalamic luteinizing hormone-releasing hormone (LHRH) neurons, LHRH and a higher immunoreactive molecular form (MW 1,800 daltons) of the decapeptide were immunoassayed with antibodies of different specificities in hypothalamic subcellular fractions, after molecular sieve filtration on Biogel P4 columns equilibrated with 0.2 N acetic acid containing 0.02% sodium azide. The study was performed in ovariectomized (OVX), ovariectomized estradiol-implanted (OVX + E2) or OVX + E2 progesterone one-treated rats (OVX + E2 + P). The animals were killed before or during the circadian luteinizing hormone (LH) surge. The amount of LHRH-like immunoreactivity recovered from the synaptosomal fraction was slightly increased in OVX + E2-implanted animals but very markedly augmented in OVX + E2 + P-treated rats. In contrast, the higher molecular form recovered from a high-speed supernatant was markedly decreased in OVX + E2 + P-treated rats when compared to the other groups. At the time of maximal LH release induced by E2 + P administration, hypothalamic LHRH was markedly depleted, whereas the larger molecular form was notably augmented. The data suggest that ovarian steroids not only influence release of hypothalamic LHRH but also the processing of LHRH precursor forms.