Community-acquired Clostridium difficile diarrhea caused by binary toxin, toxin A, and toxin B gene-positive isolates in Hungary
Community-acquired Clostridium difficile diarrhea caused by binary toxin, toxin A, and toxin B gene-positive isolates in Hungary
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DOI：
10.1128/jcm.42.9.4316-4318.2004
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发表时间：
2004-09-01
影响因子：
9.4
通讯作者：
Nagy, E
中科院分区：
文献类型：
--
作者：
Terhes, G;Urb치n, E;Nagy, E
这项工作的目的是研究艰难梭菌菌株的毒素类型来自匈牙利的不同地区。用PCR方法扩增两个主要毒素基因和二元毒素基因，并检测毒素A基因3'端的缺失或插入。将结果与HeLa细胞系的细胞毒性测定结果进行了比较。对112株分离株进行了检测，其中79株用PCR方法检测到毒素A和毒素B基因。所有PCR法阳性的分离株细胞毒性试验也呈阳性。所有其他菌株（n = 33）的毒素A和毒素B基因均为阴性;在这些情况下，未观察到对细胞系的细胞病变效应。PCR方法未检出tcd 4阴性和tcdB阳性的分离株。在两种情况下，通过PCR观察到二元毒素基因的存在;从粪便中分离的两种分离株均携带tcd 4和tcdB基因。两例病例均未发生既往住院治疗。
The aim of this work was to study the toxin types of Clostridium difficile isolates originating from different parts of Hungary. A PCR method was used for amplification of the two major toxin genes and the binary toxin gene and to detect the deletion or insertion in the 3' end of the toxin A gene. The findings were compared with the results of cytotoxicity assays on the HeLa cell line. One hundred twelve isolates were tested; the toxin A and toxin B genes were detected in 79 strains by the PCR method. All of the isolates that were positive by the PCR method were also positive by the cytotoxicity assay. All of the other strains (n = 33) were negative for the toxin A and toxin B genes; in these cases, cytopathic effects on the cell line were not observed. No tcd4-negative and tcdB-positive isolates were found by the PCR method. In two cases, the presence of a binary toxin gene was observed by PCR; both isolates that were isolated from diarrheal feces carried the tcd4 and tcdB genes. No prior hospitalization had occurred in either case.