Detection of Serum Gastric Cancer-Associated MG7-Ag from Gastric Cancer Patients Using a Sensitive and Convenient ELISA Method
Detection of Serum Gastric Cancer-Associated MG7-Ag from Gastric Cancer Patients Using a Sensitive and Convenient ELISA Method
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使用灵敏且方便的 ELISA 方法检测胃癌患者血清中胃癌相关 MG7-Ag
DOI：
10.1080/07357900802175609
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发表时间：
2009-01-01
影响因子：
2.4
通讯作者：
Fan, Daiming
中科院分区：
文献类型：
--
作者：
Jin, Bin;Wang, Xin;Fan, Daiming
目的：采用简便的ELISA方法检测胃癌患者及部分对照组血清中的MG7-Ag，寻求一种高灵敏度、简便的胃癌早期诊断方法。实验设计：采用简便的ELISA方法检测116例术前胃癌患者、63例胃癌术后患者、78例癌前病变患者、50例健康献血者和其他癌症患者血清MG7-Ag的表达。为了进行比较，还检测了术前胃癌患者的血清CEA、CA 50、CA 19-9和TAG-72。同时采用免疫组化方法检测上述胃癌及癌前病变组中MG7-Ag的表达情况。结果：ELISA法测定Mg7-Ag阳性率为83。术前胃癌患者6%，肺癌患者54.8%，直肠癌患者45.5%，结肠癌患者17.6%，乳腺癌患者14.2%，术后胃癌患者47.6%，癌前病变患者12.8%，健康献血者0%。 ELISA 的敏感性 (83.6%) 与免疫组化 (94%, p > 0. 01) 相似，但假阳性率较低 (12.8% vs. 51.3%)。胃癌中 MG7-Ag 的表达水平与肿瘤分化程度 (p < 0. 01) 和病理分期 (p < 0. 01) 相关。结论：该ELISA方法可能是筛查大量胃癌高危人群的无创候选方法。
Purpose: To seek a high sensitive and convenient method for early diagnosis of gastric cancer by testing MG7-Ag in serum of gastric cancer patients and some other control groups using a convenient ELISA method. Experiment Design: The expression of serum MG7-Ag was detected in 116 preoperative gastric cancer patients, 63 postoperative gastric cancer patients, 78 precancerous lesion patients, 50 healthy blood donors and patients of other cancers by a convenient ELISA method. For comparison, serum CEA, CA 50, CA 19-9 and TAG-72 were also detected in preoperative gastric cancer patients. Meanwhile, the expression of MG7-Ag was detected by immunohistochemical analysis in the groups of patients with gastric cancer or precancerous lesion mentioned above. Results: The positive rate of Mg7-Ag determined by ELISA was 83. 6% of preoperative gastric cancer patients, 54.8% of lung cancer patients, 45.5% of rectal cancer patients, 17.6% of colonic cancer patients, 14.2% of breast cancer patients, 47.6% of postoperative gastric cancer patients, 12.8% of precancerous lesions patients and 0% of healthy blood donors, respectively. The sensitivity of ELISA (83.6%) was found to be similar with that of immunohistochemistry (94%, p > 0. 01), while the false positive rate was lower (12.8% vs. 51.3%). MG7-Ag expression level in gastric cancer was correlated with tumor differentiation (p < 0. 01) and pathological stage (p < 0. 01). Conclusion: This ELISA method may be a non-invasive candidate method for screening of large population with high risk of gastric cancer.