INDUCED MUSCLE DIFFERENTIATION IN AN EMBRYONAL CARCINOMA CELL-LINE
INDUCED MUSCLE DIFFERENTIATION IN AN EMBRYONAL CARCINOMA CELL-LINE
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DOI：
10.1128/mcb.3.12.2280
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发表时间：
1983-01-01
影响因子：
5.3
通讯作者：
MCBURNEY, MW
中科院分区：
文献类型：
--
作者：
EDWARDS, MKS;HARRIS, JF;MCBURNEY, MW
[小鼠]畸胎瘤衍生系P19 S1801 A1（01 A1）的细胞是多能胚胎癌细胞，当聚集并暴露于二甲基亚砜时可诱导分化。许多非神经细胞类型出现在二甲基亚砜处理的文化，心肌和骨骼肌是最容易识别。使用针对肌球蛋白的单克隆抗体的免疫荧光程序，以确认和定量形成的肌细胞数量。与胚胎癌特异性表面抗原反应的单克隆抗体用于确认二甲基亚砜处理后未分化细胞的消失。心肌细胞在药物暴露的4-5天内发育，但骨骼肌细胞直到7-8天才变得明显。分离出了一种突变细胞系（D3），它似乎不能发育肌肉，但当暴露于高浓度视黄酸时，它能形成神经元和神经胶质细胞。该系统可能是有用的调查的手段，其中多能细胞成为致力于发展沿着横纹肌谱系。
Cells of the [mouse] teratocarcinoma-derived line P19S1801A1 (01A1) are pluripotent embryonal carcinoma cells and can be induced to differentiate when aggregated and exposed to dimethyl sulfoxide. Many nonneural cell types appear in dimethyl sulfoxide-treated cultures, cardiac and skeletal muscle being the most easily identified. Immunofluorescence procedures with monoclonal antibodies directed against muscle myosin to confirm and quantitate the number of muscle cells formed. A monoclonal antibody reactive with an embryonal carcinoma-specific surface antigen was used to confirm the disappearance of undifferentiated cells after dimethyl sulfoxide treatment. Cardiac muscle cells developed within 4-5 days of drug exposure, but skeletal muscle cells did not become evident until 7-8 days. A mutant cell line (D3) which appears to be incapable of muscle development but which does form neurons and glial cells when exposed to high retinoic acid concentrations was isolated. This system may be useful for investigation of the means by which pluripotent cells become committed to development along the striated muscle lineages.