A highly polymorphic cDNA probe in the NF1 gene.
A highly polymorphic cDNA probe in the NF1 gene.
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NF1 基因中的高度多态性 cDNA 探针。
DOI：
10.1093/nar/19.13.3754
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发表时间：
1991
影响因子：
14.9
通讯作者：
Collins,FS
中科院分区：
文献类型：
--
作者：
Andersen,LB;Wallace,MR;Marchuk,DA;Tavakkol,R;Mitchell,A;Saulino,AM;Collins,FS
来源及说明：一个2.3kb的cDNA克隆，AE25（1），通过用P5筛选人胎脑cDNA文库（Stratagene，目录号936206）获得，P5是1型神经纤维瘤病（NF 1）基因3 '翻译区的cDNA克隆（2）。将插入片段亚克隆到Bluescript.Polymorphisms的EcoRI位点和频率：用四种酶之一消化基因组DNA揭示多态性，如下所示。在不同的RFLP之间，特别是在由BglII和PvuII鉴定的多态性之间，似乎存在强烈的连锁不平衡。非多态性：Hincd和XbaI（分别在7和19个个体中研究）。
Source and Description: A 2.3 kb cDNA clone, AE25 (1), obtained by screening a human fetal brain cDNA library (Stratagene, catalog number 936206), with P5, a cDNA clone in the 3'translated region of the neurofibromatosis type 1 (NF1) gene (2). The insert was subcloned into the EcoRI site of Bluescript.Polymorphisms and Frequencies: Digestion of genomic DNA with one of four enzymes reveals polymorphism, listed schematically below. There appears to be strong linkage disequilibrium between the different RFLP's, particularly between the polymorphisms identified by BglII and PvuII. Not Polymorphic For: Hincd and XbaI (studied in 7 and 19 individuals, respectively).