The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating
The role of Far1p in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating
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DOI：
10.1126/science.282.5393.1511
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发表时间：
1998-11-20
期刊：
影响因子：
56.9
通讯作者：
Peter, M
中科院分区：
文献类型：
--
作者：
Butty, AC;Pryciak, PM;Peter, M
异源三聚体三磷酸鸟苷（GTP）结合蛋白（G蛋白）决定各种生物体中的组织和细胞极性。在酵母中，细胞通过一种需要Far1p和Cdc24 p的机制，沿着沿着一种信息素梯度向交配伴侣定向极化生长。Far1p结合G β γ，并与极性建立蛋白相互作用，这些蛋白组织肌动蛋白细胞骨架。含有突变的Far1p的细胞不能结合G β γ或极性建立蛋白，因此无法向其交配伴侣定向生长。响应于信息素，Far1p从细胞核移动到细胞质，因此，Far1p作为一个适配器，将极性建立蛋白募集到由G β γ标记的细胞外信号传导位点，以形态发生梯度促进细胞骨架的组装。
Heterotrimeric guanosine triphosphate (GTP)-binding proteins (G proteins) determine tissue and cell polarity in a variety of organisms. In yeast, cells orient polarized growth toward the mating partner along a pheromone gradient by a mechanism that requires Far1p and Cdc24p. Far1p bound G beta gamma and interacted with polarity establishment proteins, which organize the actin cytoskeleton. Cells containing mutated Far1p unable to bind G beta gamma or polarity establishment proteins were defective for orienting growth toward their mating partner. In response to pheromones, Far1p moves from the nucleus to the cytoplasm, Thus, Far1p functions as an adaptor that recruits polarity establishment proteins to the site of extracellular signaling marked by G beta gamma to polarize assembly of the cytoskeleton in a morphogenetic gradient.