Isolation, purification, and growth of human skeletal muscle cells.
Isolation, purification, and growth of human skeletal muscle cells.
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人类骨骼肌细胞的分离、纯化和生长。
DOI：
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发表时间：
1996
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通讯作者：
G. Pavlath
中科院分区：
文献类型：
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作者：
G. Pavlath
骨骼肌细胞可在体外用于肌生成素研究，也可在 VIVO 中用作治疗肌肉和非肌肉疾病的基因传递载体 (1-9)。这些骨骼肌细胞源自肌肉卫星细胞，位于分化肌纤维的基底层和肌膜之间 (10) 在胎儿期和产后早期的肌肉发育和生长期后通常处于静止状态，这些细胞在肌肉损伤时被诱导增殖并与现有肌肉纤维融合。体外分离和生长的卫星细胞称为成肌细胞。成肌细胞在富含有丝分裂原的培养基中增殖，但在达到高细胞密度后暴露于肌肉分化的特征是成肌细胞融合形成多核肌管，表达分化特异性蛋白。本章给出了使用流式细胞术 (11) 和细胞克隆 (12-14) 两种不同技术从人体肌肉组织中分离成肌细胞的方法。这些方法适用于胎儿和产后捐赠者以及正常和患病个体的肌肉组织。
Skeletal muscle cells can be used in vitro for the study of myogenests, as well as in VIVO as gene-delivery vehicles for the therapy of muscle and nonmuscle diseases (1-9). These skeletal muscle cells are derived from muscle satellite cells, which he between the basal lamina and the sarcolemma of differentiated muscle fibers (10) Normally quiescent after the period of muscle development and growth during fetal life and the early postnatal period, these cells are induced to proliferate on muscle damage and fuse with existing muscle fibers Satellite cells isolated and grown in vitro are called myoblasts Myoblasts proliferate in mitogen-rich media, but on reaching high cell density followed by exposure to mitogen-poor media, are induced to differentiate and become postmitotic Muscle differentiation is characterized by the fusion of myoblasts to form multinucleated myotubes, which express differentiation-specific proteins. In this chapter, methods are given for the isolation of myoblasts from human muscle tissue using two different techinques flow cytometry (11) and cell cloning (12-14). These methods are applicable to muscle tissue from both fetal and postnatal donors, as well as from normal and diseased individuals.