The plant gene CCD1 selectively blocks cell death during the hypersensitive response to cauliflower mosaic virus infection
The plant gene CCD1 selectively blocks cell death during the hypersensitive response to cauliflower mosaic virus infection
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DOI：
10.1094/mpmi-18-0212
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发表时间：
2005-03-01
影响因子：
3.5
通讯作者：
Schoelz, JE
中科院分区：
文献类型：
--
作者：
Cawly, J;Cole, AB;Schoelz, JE
花椰菜花叶病毒（CaMV）W260的P6蛋白在烟草（Nicotiana edwardsonii）上引起过敏反应。这种防御反应，常见于许多植物病原体，具有两个关键特征，即最初感染组织内的细胞死亡和病原体对该区域的限制。我们提出的证据表明，一个植物基因命名为CCD 1，最初确定在N。bigelovii可以选择性地阻断HR期间的细胞死亡途径，而针对W260的抗性途径保持完整。细胞死亡的抑制不仅在宏观上而且在微观上都是明显的。HR介导的细胞死亡的抑制对CaMV是特异性的，因为烟草花叶病毒能够在含有CCD的植物中引发HR]。CCD]还阻断了由N.克莱维兰地。从N. bigelovii转化为N. clevelandii能阻断W260感染引起的系统性细胞死亡，但不能阻止番茄丛矮病毒诱导的系统性细胞死亡。因此，CCD1阻断由W260的P6诱导的局部和系统性细胞死亡，但不作为由其他植物病毒诱导的细胞死亡的一般抑制剂。此外，用CCD 1进行的实验提供了进一步的证据，表明细胞死亡可以与爱德华氏烟草HR对CaMV W260的抗性解偶联。
The P6 protein of Cauliflower mosaic virus (CaMV) W260 elicits a hypersensitive response (HR) on inoculated leaves of Nicotiana edwardsonii. This defense response, common to many plant pathogens, has two key characteristics, cell death within the initially infected tissues and restriction of the pathogen to this area. We present evidence that a plant gene designated CCD1, originally identified in N. bigelovii, can selectively block the cell death pathway during HR, whereas the resistance pathway against W260 remains intact. Suppression of cell death was evident not only macroscopically but also microscopically. The suppression of HR-mediated cell death was specific to CaMV, as Tobacco mosaic virus was able to elicit HR in the plants that contained CCD]. CCD] also blocks the development of a systemic cell death symptom induced specifically by the P6 protein of W260 in N. clevelandii. Introgression of CCD1 from N. bigelovii into N. clevelandii blocked the development of systemic cell death in response to W260 infection but could not prevent systemic cell death induced by Tomato bushy stunt virus. Thus, CCD1 blocks both local and systemic cell death induced by P6 of W260 but does not act as a general suppressor of cell death induced by other plant viruses. Furthermore, experiments with CCD1 provide further evidence that cell death could be uncoupled from resistance in the HR of Nicotiana edwardsonii to CaMV W260.