Cell surface topology creates high Ca2+ signalling microdomains.
Cell surface topology creates high Ca2+ signalling microdomains.
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DOI：
10.1016/j.ceca.2010.01.005
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发表时间：
2010-04
期刊：
影响因子：
4
通讯作者：
Hallett MB
中科院分区：
文献类型：
--
作者：
Brasen JC;Olsen LF;Hallett MB
长期以来，人们一直推测细胞微区对许多细胞过程，特别是涉及Ca2+信号传导的细胞过程是重要的。细胞溶质Ca2+的测量报告最大浓度小于几微摩尔，但几种细胞溶质酶需要超过20 μM的Ca2+浓度才能被激活。在本文中，我们已经解决了这一明显的矛盾，表明细胞的表面拓扑结构代表了一个重要的和迄今为止尚未认识到的功能，产生高钙微区的细胞。我们发现，而一个光滑的细胞表面的标准建模假设预测只有适度的局部效应，更现实的“褶皱”的表面拓扑结构预测，Ca2+浓度高达80 μM可以持续在膜的褶皱内的显着时间。该皱纹内位置可占总细胞体积的5%。使用不同的几何形状的皱纹，我们的模拟表明，高Ca2+微域将产生最有效的长窄膜皱纹的尺寸相似的实验发现。这是一个新的概念，以前没有考虑过，但它有分歧的皱纹内的位置也是一个战略位置，其中Ca2+作为调节皮质细胞骨架和质膜扩张。
It has long been speculated that cellular microdomains are important for many cellular processes, especially those involving Ca2+ signalling. Measurements of cytosolic Ca2+ report maximum concentrations of less than few micromolar, yet several cytosolic enzymes require concentrations of more than 20 μM Ca2+ to be activated. In this paper, we have resolved this apparent paradox by showing that the surface topology of cells represents an important and hitherto unrecognized feature for generating microdomains of high Ca2+ in cells. We show that whereas the standard modeling assumption of a smooth cell surface predicts only moderate localized effects, the more realistic “wrinkled” surface topology predicts that Ca2+ concentrations up to 80 μM can persist within the folds of membranes for significant times. This intra-wrinkle location may account for 5% of the total cell volume. Using different geometries of wrinkles, our simulations show that high Ca2+ microdomains will be generated most effectively by long narrow membrane wrinkles of similar dimensions to those found experimentally. This is a new concept which has not previously been considered, but which has ramifications as the intra-wrinkle location is also a strategic location at which Ca2+ acts as a regulator of the cortical cytoskeleton and plasma membrane expansion.
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影响因子：
3.2
作者：
Bruehl, RE;Springer, TA;Bainton, DF
通讯作者：
Bainton, DF
影响因子：
4
作者：
Davies, EV;Hallett, MB
通讯作者：
Hallett, MB
影响因子：
64.8
作者：
Finch, EA;Augustine, GJ
通讯作者：
Augustine, GJ
DOI：
10.1073/pnas.83.9.2919
发表时间：
1986-05-01
影响因子：
11.1
作者：
KRUSKAL, BA;SHAK, S;MAXFIELD, FR
通讯作者：
MAXFIELD, FR
影响因子：
64.8
作者：
Fernández-Chacón, R;Königstorfer, A;Südhof, TC
通讯作者：
Südhof, TC