MARKED INCREASE OF HGF MESSENGER-RNA IN NON-PARENCHYMAL LIVER-CELLS OF RATS TREATED WITH HEPATOTOXINS
MARKED INCREASE OF HGF MESSENGER-RNA IN NON-PARENCHYMAL LIVER-CELLS OF RATS TREATED WITH HEPATOTOXINS
复制标题
DOI：
10.1016/0006-291x(89)92733-2
复制
发表时间：
1989-12-29
影响因子：
3.1
通讯作者：
NAKAMURA, T
中科院分区：
文献类型：
--
作者：
KINOSHITA, T;TASHIRO, K;NAKAMURA, T
CCl4、d -半乳糖胺等肝毒素诱导大鼠实验性肝炎后，肝组织中HGF mRNA显著升高。HGF mRNA的升高具有时间和剂量依赖性。ccl4治疗后5小时。HGF mRNA显著升高；它在10小时达到最大水平，并保持在这个水平40小时。相反，在d -半乳糖胺诱导的肝炎中，HGF mRNA在较长滞后时间后从24小时开始升高。HGF mRNA的表达是短暂的，在36小时达到最大值后迅速下降到基础水平。HGF mRNA的诱导程度与肝损伤程度密切相关。在肝脏中，HGF mRNA仅在非实质细胞中检测到，而在实质肝细胞中未检测到。这些结果提示肝脏是肝损伤后肝再生的主要HGF产生器官，HGF由非实质肝细胞合成和分泌，刺激实质肝细胞生长，以旁分泌方式修复肝组织。
When experimental hepatitis was induced by administrating rats with hepatotoxins such as CCl4 and D-galactosamine, HGF mRNA increased dramatically in the injured liver. The increase of HGF mRNA was time- and dose-dependent. At 5 hr after CCl4-treatment. HGF mRNA was remarkably increased; it reached the maximum level at 10 hr and maintained at this level for 40 hr. On the contrary, in D-galactosamine-induced hepatitis, HGF mRNA started to increase from 24 hr after a long lag time. Moreover, HGF mRNA was expressed transiently, decreasing rapidly to the basal level after reaching the maximum level at 36 hr. The degree of induction of HGF mRNA correlates well to the degree of liver damage. In the liver, HGF mRNA could be detected in only non-parenchymal cells, not in parenchymal hepatocytes. These findings suggest that liver is a main producing organ of HGF for liver regeneration after hepatic injury, and HGF is synthesized and secreted by non-parenchymal liver cells so that it stimulates the growth of parenchymal hepatocytes to repair liver tissue in paracrine fashion.