Direct, simplified, and sensitive assay of angiotensin II in plasma extracts performed with a high-affinity monoclonal antibody.
Direct, simplified, and sensitive assay of angiotensin II in plasma extracts performed with a high-affinity monoclonal antibody.
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使用高亲和力单克隆抗体对血浆提取物中的血管紧张素 II 进行直接、简化且灵敏的测定。
DOI：
10.1093/clinchem/38.10.1963
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发表时间：
1992
影响因子：
9.3
通讯作者：
P. Corvol
中科院分区：
文献类型：
--
作者：
D. Simon;B. Romestand;H. Huang;G. Badouaille;J. Fehrentz;B. Pau;J. Marchand;P. Corvol
基于一种具有高亲和力和特异性的单克隆抗体，建立了一种非常简单、快速、灵敏的血管紧张素II（Ang II）放射免疫分析方法，使固相萃取后直接测定人血浆中循环Ang II成为可能。纯化的单克隆抗体4D 8与Ang II的结合常数为1.3 × 10（11）L/mol，与Ang I的交叉反应性< 1%。该测定法可以检测到2 mL血浆中低至0.8fmol的Ang II，并且不受Ang I存在的影响。在112%和116%之间的分析回收率获得的血管紧张素II添加到人血浆中的生理浓度。比较RIA与反相，高效液相色谱法，然后用RIA测量正常和高血压受试者的人血浆样品中的Ang II-和正常血压受试者之前和之后的急性抑制血管紧张素转换酶与卡托普利（50毫克）-显示了高度的相关性（r2 = 0.93）之间的两种方法。
A very simple, fast, and sensitive RIA of angiotensin (Ang) II has been developed, based on a monoclonal antibody with high affinity and specificity, making possible the direct measurement of circulating Ang II in human plasma after solid-phase extraction. The purified monoclonal antibody 4D8 has an association constant of 1.3 x 10(11) L/mol with Ang II and a cross-reactivity of < 1% for Ang I. The assay can detect as little as 0.8 fmol of Ang II in 2 mL of plasma and is not influenced by the presence of Ang I. Analytical recoveries between 112% and 116% were obtained for Ang II added to human plasma at physiological concentrations. Comparison of the RIA with a reversed-phase, high-performance liquid chromatographic method followed by RIA to measure Ang II in human plasma samples from normal and hypertensive subjects--and from normotensive subjects before and after an acute inhibition of angiotensin-converting enzyme with captopril (50 mg)--showed a high degree of correlation (r2 = 0.93) between the two methods.