Revealing Clonal Responses of Tumor-Reactive T-Cells Through T Cell Receptor Repertoire Analysis.
Revealing Clonal Responses of Tumor-Reactive T-Cells Through T Cell Receptor Repertoire Analysis.
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DOI：
10.3389/fimmu.2022.807696
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发表时间：
2022
影响因子：
7.3
通讯作者：
Ueha S
中科院分区：
文献类型：
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作者：
Aoki H;Shichino S;Matsushima K;Ueha S
CD8+ T细胞是参与抗肿瘤免疫应答的关键效应细胞。它们包括具有不同抗原特异性T细胞受体（TCR）的各种T细胞克隆。因此，阐明不同T细胞克隆的整体抗肿瘤反应是肿瘤免疫学中的一个新兴挑战。随着新一代DNA测序仪的发展，全面分析TCR基因的集合（TCR库分析）是可行的，并已被用于研究抗肿瘤T细胞的克隆反应。然而，TCR库指数的免疫病理学意义仍不明确。在这篇综述中，我们介绍了两种方法，促进免疫学解释的TCR库数据：器官间克隆跟踪分析和单细胞TCR测序。这些用于TCR库分析的方法将提供对肿瘤微环境中肿瘤特异性T细胞的反应的更准确的理解。
CD8+ T cells are the key effector cells that contribute to the antitumor immune response. They comprise various T-cell clones with diverse antigen-specific T-cell receptors (TCRs). Thus, elucidating the overall antitumor responses of diverse T-cell clones is an emerging challenge in tumor immunology. With the recent advancement in next-generation DNA sequencers, comprehensive analysis of the collection of TCR genes (TCR repertoire analysis) is feasible and has been used to investigate the clonal responses of antitumor T cells. However, the immunopathological significance of TCR repertoire indices is still undefined. In this review, we introduce two approaches that facilitate an immunological interpretation of the TCR repertoire data: inter-organ clone tracking analysis and single-cell TCR sequencing. These approaches for TCR repertoire analysis will provide a more accurate understanding of the response of tumor-specific T cells in the tumor microenvironment.