Genetic analysis of tumorigenesis: I. Expression of tumor-forming ability in hamster hybrid cell lines
Genetic analysis of tumorigenesis: I. Expression of tumor-forming ability in hamster hybrid cell lines
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肿瘤发生的遗传分析：一、仓鼠杂交细胞系成瘤能力的表达
DOI：
10.1007/bf01542849
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
P. Kovac
中科院分区：
文献类型：
--
作者：
R. Sager;P. Kovac
描述了两种新的中国雄性仓鼠胚胎成纤维细胞系：一种在裸鼠中具有致瘤性（CHEF 16），另一种不具有致瘤性（CHEF 18-1）。两系均具有稳定的二倍体模式，平均为22对，有10对同源常染色体。这些细胞系在甲基纤维素的克隆能力、菌落形态和致瘤性方面存在明显差异；在一组18-1 × 16-2杂交无性系中检测了这些性状的表达。结果显示，最初抑制了致瘤性和锚定独立性，随后在裸鼠中生长了染色体减少到二倍体范围的细胞亚群。进一步的研究正在进行中，以确定甲基纤维素和裸鼠的生长能力是否在细胞水平上协调分离，以及这种分离是否具有可识别的染色体基础。
Two new fibroblast cell lines from a male Chinese hamster embryo are described: one tumorigenic in nude mice (CHEF 16) and the other not (CHEF 18-1). Both lines have a stable diploid mode and mean of 22 with 10 pairs of homologous autosomes. The cell lines differ unambiguously in cloning ability in methylcellulose, colony morphology, and tumorigenicity; the expression of these traits was examined in a set of 18-1 × 16-2 hybrid clones. The results show initial suppression of tumorigenicity and anchorage independence, followed by growth in the nude mouse of subsets of cells with chromosomes reduced to the diploid range. Further studies are in progress to establish whether ability to grow in methylcellulose and in the nude mouse segregate coordinately at the cellular level, and whether this segregation has an identifiable chromosomal basis.