An Atlas of Vagal Sensory Neurons and Their Molecular Specialization
An Atlas of Vagal Sensory Neurons and Their Molecular Specialization
复制标题
DOI：
10.1016/j.celrep.2019.04.096
复制
发表时间：
2019-05-21
期刊：
影响因子：
8.8
通讯作者：
Ernfors, Patrik
中科院分区：
文献类型：
--
作者：
Kupari, Jussi;Haring, Martin;Ernfors, Patrik
迷走神经的感觉功能对于意识感知和监测心肺和胃肠系统的内脏功能至关重要。在这里，我们通过单细胞RNA测序（scRNA-seq）转录组学分析，对神经嵴（颈静脉）和基板（结节）来源的迷走神经节中的神经元类型进行了全面的鉴定、分类和验证。我们的研究结果揭示了不同胚胎来源的神经元之间的主要差异。颈静脉神经元与体感觉脊髓神经元具有基本的相似性，包括主要类型，如c -低阈机械感受器（C-LTMRs）、A- ltmrs、A- δ伤害感受器、冷-和机械-热- c伤害感受器。相反，结节神经节包含18种不同的类型，专门用于测量体内的生理状态。我们的研究结果揭示了迷走神经神经元类型的巨大多样性，包括许多以前未预料到的类型，以及与化学感受器、营养检测器、压力感受器、呼吸系统、胃肠道和心血管系统的拉伸和体积机械感受器一致的建议类型。
Sensory functions of the vagus nerve are critical for conscious perceptions and for monitoring visceral functions in the cardio-pulmonary and gastrointestinal systems. Here, we present a comprehensive identification, classification, and validation of the neuron types in the neural crest (jugular) and placode (nodose) derived vagal ganglia by single-cell RNA sequencing (scRNA-seq) transcriptomic analysis. Our results reveal major differences between neurons derived from different embryonic origins. Jugular neurons exhibit fundamental similarities to the somatosensory spinal neurons, including major types, such as C-low threshold mechanoreceptors (C-LTMRs), A-LTMRs, A delta-nociceptors, and cold-, and mechano-heat C-nociceptors. In contrast, the nodose ganglion contains 18 distinct types dedicated to surveying the physiological state of the internal body. Our results reveal a vast diversity of vagal neuron types, including many previously unanticipated types, as well as proposed types that are consistent with chemoreceptors, nutrient detectors, baroreceptors, and stretch and volume mechanoreceptors of the respiratory, gastrointestinal, and cardiovascular systems.