In vitro study of biofilm formation and effectiveness of antimicrobial treatment on various dental material surfaces
In vitro study of biofilm formation and effectiveness of antimicrobial treatment on various dental material surfaces
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DOI：
10.1111/j.2041-1014.2010.00586.x
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发表时间：
2010-12-01
影响因子：
3.7
通讯作者：
Shi, W.
中科院分区：
文献类型：
--
作者：
Li, L.;Finnegan, M. B.;Shi, W.
义齿上形成的生物膜中白色念珠菌的比例升高与口腔炎有关，而变形链球菌在修复材料上的积累可引起继发性龋。白色念珠菌、S.变异株、唾液来源的和C.白念珠菌/唾液来源的混合生物膜生长在不同的材料上，包括丙烯酸义齿、瓷、羟基磷灰石（HA）和聚苯乙烯。通过三维图像量化和菌落形成单位评估来分析所得到的生物量。还通过菌落计数评估了用溶解的义齿清洁片（Polident（R））进行生物膜处理的功效。HA上形成的生物膜在生物量积累方面表现出最显著的差异：唾液细菌生物膜积累的总生物量最高，而C。与其他材料（包括丙烯酸义齿表面）相比，HA表面的白色念珠菌生物膜形成大大减少。这些结果证实了临床发现，丙烯酸义齿可以包括一个水库的C。白色念珠菌，这使得患者更容易受到C。白色念珠菌感染和口腔炎。此外，相同类型的生物膜的处理功效根据表面而显著变化。虽然在聚苯乙烯表面上形成的单一物种生物膜表现出对处理的最高敏感性，但对于所有类型的测试生物膜，从HA表面回收的存活细胞最多。这项研究表明，表面的性质影响生物膜的特性，包括生物量的积累和敏感性抗菌处理。因此，应在目标病原体定殖的表面上评价此类处理。
P>Elevated proportions of Candida albicans in biofilms formed on dentures are associated with stomatitis whereas Streptococcus mutans accumulation on restorative materials can cause secondary caries. Candida albicans, S. mutans, saliva-derived and C. albicans/saliva-derived mixed biofilms were grown on different materials including acrylic denture, porcelain, hydroxyapatite (HA), and polystyrene. The resulting biomass was analysed by three-dimensional image quantification and assessment of colony-forming units. The efficacy of biofilm treatment with a dissolved denture cleansing tablet (Polident (R)) was also evaluated by colony counting. Biofilms formed on HA exhibited the most striking differences in biomass accumulation: biofilms comprising salivary bacteria accrued the highest total biomass whereas C. albicans biofilm formation was greatly reduced on the HA surface compared with other materials, including the acrylic denture surface. These results substantiate clinical findings that acrylic dentures can comprise a reservoir for C. albicans, which renders patients more susceptible to C. albicans infections and stomatitis. Additionally, treatment efficacy of the same type of biofilms varied significantly depending on the surface. Although single-species biofilms formed on polystyrene surfaces exhibited the highest susceptibility to the treatment, the most surviving cells were recovered from HA surfaces for all types of biofilms tested. This study demonstrates that the nature of a surface influences biofilm characteristics including biomass accumulation and susceptibility to antimicrobial treatments. Such treatments should therefore be evaluated on the surfaces colonized by the target pathogen(s).