Transcriptional regulation of an unusual trypsin-related protein expressed during insect metamorphosis.
Transcriptional regulation of an unusual trypsin-related protein expressed during insect metamorphosis.
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昆虫变态过程中表达的异常胰蛋白酶相关蛋白的转录调控。
DOI：
10.1016/0965-1748(93)90071-y
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发表时间：
1993
影响因子：
3.8
通讯作者：
Manczak,M
中科院分区：
文献类型：
--
作者：
Jones,G;Venkataraman,V;Manczak,M
获得了在幼虫-蛹变态期间表达的胰蛋白酶相关蛋白的全长cDNA。编码的蛋白质N末端一半与胰蛋白酶蛋白酶具有相似性，包括催化三联体中正确定位的组氨酸和天冬氨酸成员。然而，编码蛋白质的其余部分与胰蛋白酶几乎没有相似之处，并且完全缺失含有催化丝氨酸残基的典型序列。体外转录/翻译产物为28kDa的蛋白质。在保幼激素滴度下降的正常条件下，转录的mRNA作为总基因组转录的比例稳步增加的前3天的最后体育场，但这种增加被延迟和抑制维持高保幼激素滴度。在转录的正常增加的第3天的最后体育场，急剧下降，观察到在稳态丰度的转录本，测量相对于剩余的mRNA的丰度，这表明该消息的稳定性降低后，组织承诺蛹蜕皮。
The full-length cDNA for a trypsin-related protein expressed during larval-pupal metamorphosis was obtained. The encoded N-terminal half of the protein possessed similarity to trypsin proteases, including the correctly positioned histidine and aspartic acid members of the catalytic triad. However, the remainder of the encoded protein bore little resemblance to trypsin, and was altogether missing the canonical sequence containing the catalytic serine residue. The product ofin vitrotranscription/translation of the cDNA was a 28 kDa protein. Under normal conditions of a declining juvenile hormone titer, transcription of the mRNA as a proportion of total genomic transcription steadily increased for the first 3 days of the final stadium, but this increase was delayed and suppressed by maintenance of a high juvenile hormone titer. During the normal increase in transcription on day 3 of the final stadium, a sharp decline was observed in the steady-state abundance of the transcript, measured relative to abundance of the remaining mRNAs, suggesting that the stability of the message decreases after tissue commitment for a pupal molt.