Regionalized expression of myosin isoforms in heterotypic myotubes formed from embryonic and fetal rat myoblasts in vitro
Regionalized expression of myosin isoforms in heterotypic myotubes formed from embryonic and fetal rat myoblasts in vitro
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体外胚胎和胎鼠成肌细胞形成的异型肌管中肌球蛋白亚型的区域化表达
DOI：
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发表时间：
1997
影响因子：
2.5
通讯作者：
P. Merrifield
中科院分区：
文献类型：
--
作者：
C. Pin;P. Merrifield
哺乳动物肢体肌肉的发育涉及至少两个独立的肌肉前体细胞群的出现和融合。这两个群体被称为胚胎和胎儿成肌细胞，首先在不同发育阶段的肢芽内检测到。我们之前已经证明，在大鼠中，每个成肌细胞群在体外分化过程中表达独特的肌球蛋白重链（MyHC）模式（Pin 和 Merrifield [1993] Dev. Genet. 14:356–368）。胚胎成肌细胞积累胚胎和慢速 MyHC，而胎儿成肌细胞则积累胚胎、新生儿和成人快速 MyHC，但不积累慢速 MyHC。为了确定两个群体是否可以相互融合以及在所得异核体中 MyHC 表达模式是否发生改变，用亲脂性染料 PKH26、[3H]-胸苷或 5-溴脱氧尿苷 (BRDU) 标记胚胎和胎儿成肌细胞，并共培养 24-48 小时。我们的结果表明胚胎和胎儿成肌细胞在体外发生融合。此外，对所得异核体的分析揭示了 MyHC 在单个细胞核周围的区域性积累。有趣的是，这些积累是单个细胞核通常在单一培养物中表达的默认表达模式的典型。由胚胎成肌细胞形成的细胞核被慢速 MyHC 的局部积累所包围，而来自胎儿成肌细胞的细胞核被新生儿/快速 MyHC 包围。这种核结构域的出现表明 MyHC 亚型的成肌细胞特异性表达是由融合前建立的顺式作用因子决定的。开发。动态。 208:420–431, 1997。© 1997 Wiley‐Liss, Inc.
The development of mammalian limb muscles involves the appearance and fusion of at least two separate populations of muscle precursor cells. These two populations, termed embryonic and fetal myoblasts, are first detected within the limb bud at different stages of development. We have previously demonstrated that, in the rat, each myoblast population expresses a unique pattern of myosin heavy chains (MyHCs) during differentiation in vitro (Pin and Merrifield [1993] Dev. Genet. 14:356–368). Embryonic myoblasts accumulate embryonic and slow MyHCs, whereas fetal myoblasts accumulate embryonic, neonatal, and adult fast MyHCs but not slow MyHC. To determine if the two populations can fuse with each other and whether the pattern of MyHC expression is altered in the resulting heterokaryons, embryonic and fetal myoblasts were labelled with the lipophilic dye PKH26, [3H]‐thymidine, or 5‐bromodeoxyuridine (BRDU) and cocultured for 24–48 hr. Our results demonstrate that fusion occurs between embryonic and fetal myoblasts in vitro. Moreover, analysis of the resulting heterokaryons revealed regionalized accumulations of MyHC around individual nuclei. Interestingly, these accumulations were typical of the default pattern of expression that individual nuclei would have normally expressed in single culture. Nuclei contributed by embryonic myoblasts were surrounded by localized accumulations of slow MyHC, whereas nuclei from fetal myoblasts were surrounded by neonatal/fast MyHC. The occurrence of such nuclear domains indicates that the myoblast‐specific expression of MyHC isoforms is dictated by cis‐acting factors established prior to fusion. Dev. Dyn. 208:420–431, 1997. © 1997 Wiley‐Liss, Inc.
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影响因子：
2.7
作者：
CONDON, K;SILBERSTEIN, L;THOMPSON, WJ
通讯作者：
THOMPSON, WJ
猫胫骨前运动单位肌肉特性的不完全神经控制的进一步证据。
DOI：
10.1152/ajpcell.1995.268.2.c527
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
Unguez,GA;Roy,RR;Pierotti,DJ;Bodine-Fowler,S;Edgerton,VR
通讯作者：
Edgerton,VR
甲基化在维持发育中肌肉中位置受限的转基因表达中的作用。
DOI：
10.1242/dev.121.7.2245
发表时间：
1995
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Grieshammer,U;McGrew,MJ;Rosenthal,N
通讯作者：
Rosenthal,N
慢肌纤维和快肌纤维优先源自在不同发育时间迁移到雏鸡肢芽中的成肌细胞。
DOI：
10.1006/dbio.1995.1218
发表时间：
1995
期刊：
Developmental biology.
影响因子：
--
作者：
VanSwearingen,J;Lance-Jones,C
通讯作者：
Lance-Jones,C
MyoD 和肌细胞生成素 mRNA 在快速和慢速成年骨骼肌中的选择性积累受到神经支配和激素的控制。
DOI：
10.1242/dev.118.4.1137
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hughes,SM;Taylor,JM;Tapscott,SJ;Gurley,CM;Carter,WJ;Peterson,CA
通讯作者：
Peterson,CA