SEVERAL MECHANISMS CAN ACCOUNT FOR DEFECTIVE E-ALPHA GENE-EXPRESSION IN DIFFERENT MOUSE HAPLOTYPES
SEVERAL MECHANISMS CAN ACCOUNT FOR DEFECTIVE E-ALPHA GENE-EXPRESSION IN DIFFERENT MOUSE HAPLOTYPES
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DOI：
10.1073/pnas.80.1.273
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
MCDEVITT, HO
中科院分区：
文献类型：
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作者：
MATHIS, DJ;BENOIST, C;MCDEVITT, HO
由主要组织相容性复合体的I区编码的鼠Ia抗原是细胞表面糖蛋白（由α组成）。和β多肽），其被认为参与免疫应答的控制。单倍型B、s、q和f的小鼠不能在细胞表面表达Ia抗原复合物之一，即E复合物。在分子水平上研究了这种缺陷（或多种缺陷）如何产生。通过使用I区E α.和A.基因探针分析RNA和DNA结构，得出的结论是，至少有3种机制可以运作。单倍型B和s的小鼠在E α中具有缺失。基因; f单倍型小鼠主要合成E α。q单倍型的小鼠具有RNA加工缺陷或mRNA稳定性问题，或两者兼而有之。
The murine Ia antigens, encoded by the I region of the major histocompatibility complex, are cell-surface glycoproteins (consisting of .alpha. and .beta. polypeptides) thought to be involved in the control of immune responsiveness. Mice of haplotypes b, s, q and f fail to express one of the Ia antigen complexes, the E complex, on the cell surface. How such a defect (or defects) might be generated was studied at the molecular level. By using I-region E.alpha. and A.alpha. gene probes for analyses of RNA and DNA structure, it was concluded that at least 3 mechanisms can operate. Mice of haplotypes b and s bear a deletion in the E.alpha. gene; f haplotype mice synthesize predominantly an E.alpha. mRNA of aberrant size; and mice of the q haplotype have a defect in RNA processing or a problem with mRNA stability, or both.