Leucine regulates α-amylase and trypsin synthesis in dairy calf pancreatic tissue in vitro via the mammalian target of rapamycin signalling pathway
Leucine regulates α-amylase and trypsin synthesis in dairy calf pancreatic tissue in vitro via the mammalian target of rapamycin signalling pathway
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DOI：
10.1017/s1751731118003683
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发表时间：
2019-09-01
期刊：
影响因子：
3.6
通讯作者：
Cao, Y. C.
中科院分区：
文献类型：
--
作者：
Guo, L.;Yao, J. H.;Cao, Y. C.
奶牛小肠中淀粉消化率低，很大程度上是由于α-淀粉酶合成不足。改善这种情况的一种策略是增强α-淀粉酶的合成。哺乳动物雷帕霉素靶蛋白（mTOR）信号通路是蛋白质合成的中心调节因子，可被亮氨酸激活。我们的目标是研究亮氨酸对mTOR信号通路的影响，并使用培养的荷斯坦奶牛小牛胰腺组织的体外模型来确定这些信号活性与胰腺酶合成之间的关联。将胰腺组织在含有L-亮氨酸的培养基中孵育3小时，每小时收集样品，包括对照但不含L-亮氨酸。亮氨酸的添加提高了α-淀粉酶和胰蛋白酶的活性及其编码基因mRNA的表达（P
Starch digestion in the small intestines of the dairy cow is low, to a large extent, due to a shortage of syntheses of alpha-amylase. One strategy to improve the situation is to enhance the synthesis of alpha-amylase. The mammalian target of rapamycin (mTOR) signalling pathway, which acts as a central regulator of protein synthesis, can be activated by leucine. Our objectives were to investigate the effects of leucine on the mTOR signalling pathway and to define the associations between these signalling activities and the synthesis of pancreatic enzymes using an in vitro model of cultured Holstein dairy calf pancreatic tissue. The pancreatic tissue was incubated in culture medium containing l-leucine for 3 h, and samples were collected hourly, with the control being included but not containing l-leucine. The leucine supplementation increased alpha-amylase and trypsin activities and the messenger RNA expression of their coding genes (P