Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function
Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function
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DOI：
10.1038/35040504
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发表时间：
2000-11-02
期刊：
影响因子：
64.8
通讯作者：
Foster, D
中科院分区：
文献类型：
--
作者：
Parrish-Novak, J;Dillon, SR;Foster, D
细胞因子在造血和免疫反应的调节中很重要，并且可以影响淋巴细胞的发育。在这里，我们已经确定了一类细胞因子受体，它在淋巴组织中选择性表达，并具有信号转导能力。全长受体在BaF3细胞中表达，建立了配体检测和克隆的功能实验。活化的人CD3(+) T细胞的条件培养基支持实验细胞系的增殖。我们从活化的人CD3+ T细胞中构建了一个互补的DNA表达文库，并利用功能克隆技术鉴定了一个具有四螺旋束结构的细胞因子。该细胞因子与IL2和IL15最密切相关，被命名为IL21，受体被命名为IL21R。体外实验表明，il - 21在骨髓自然杀伤（NK）细胞群的增殖和成熟、与抗cd40共刺激的成熟b细胞群的增殖以及与抗cd3共刺激的T细胞的增殖中发挥作用。
Cytokines are important in the regulation of haematopoiesis and immune responses, and can influence lymphocyte development. Here we have identified a class I cytokine receptor that is selectively expressed in lymphoid tissues and is capable of signal transduction. The full-length receptor was expressed in BaF3 cells, which created a functional assay for ligand detection and cloning. Conditioned media from activated human CD3(+) T cells supported proliferation of the assay cell line. We constructed a complementary DNA expression library from activated human CD3+ T cells, and identified a cytokine with a four-helix-bundle structure using functional cloning. This cytokine is most closely related to IL2 and IL15, and has been designated IL21 with the receptor designated IL21R. In vitro assays suggest that IL21 has a role in the proliferation and maturation of natural killer (NK) cell populations from bone marrow, in the proliferation of mature B-cell populations co-stimulated with anti-CD40, and in the proliferation of T cells co-stimulated with anti-CD3.