Detection of West Nile virus and Japanese encephalitis virus using real-time PCR with a probe common to both viruses
Detection of West Nile virus and Japanese encephalitis virus using real-time PCR with a probe common to both viruses
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DOI：
10.1016/j.jviromet.2005.02.001
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发表时间：
2005-06-01
影响因子：
3.1
通讯作者：
Takashima, I
中科院分区：
文献类型：
--
作者：
Shirato, K;Miyoshi, H;Takashima, I
建立了一种基于荧光实时定量聚合酶链反应（TaqMan）的西尼罗病毒（WNV）和日本脑炎病毒（JEV）鉴别诊断方法。为了用单一探针检测WNV和JEV，设计了对应于两种病毒共有的核心蛋白区域中的序列的探针。该检测方法的特异性取决于所使用的引物组，这些引物组对靶病毒序列具有特异性：WNV引物组只能检测WNV毒株，JEV引物组只能检测JEV毒株。通过检测实验感染动物组织中的病毒来测试该测定法。本研究所描述的方法将有助于同时区分西尼罗河病毒和乙脑病毒流行的地区，如东亚。(c)2005 Elsevier B.V.保留所有权利。
A diagnostic method to distinguish between West Nile virus (WNV) and Japanese encephalitis virus (JEV) based on fluorogenic real-time polymerase chain reaction (TaqMan) assays was developed. To detect WNV and JEV with a single probe, a probe was designed to correspond to sequences in the core protein region that are shared by both viruses. The specificity of this assay depended on the primer sets used, which were specific to the target virus sequences: the primer set for WNV Could detect only WNV strains and the primer set for JEV could detect only JEV strains. The assays were tested by detection of viruses from experimentally infected animal tissues. The method described in this study will be useful for the simultaneous discrimination of WNV and JEV in areas where JEV is endemic, such as East Asia. (c) 2005 Elsevier B.V. All rights reserved.