Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice
Isolation and characterization of neural stem/progenitor cells from post-stroke cerebral cortex in mice
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DOI：
10.1111/j.1460-9568.2009.06732.x
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发表时间：
2009-05-01
影响因子：
3.4
通讯作者：
Matsuyama, Tomohiro
中科院分区：
文献类型：
--
作者：
Nakagomi, Takayuki;Taguchi, Akihiko;Matsuyama, Tomohiro
在脑损伤（如中风）后，中枢神经系统具有强大的修复机制，包括内源性神经发生的激活。然而，神经干细胞/祖细胞对中风的反应尚不清楚。最近，神经干/祖细胞被发现存在于大脑皮层，以及之前发现的海马脑室下或亚颗粒区等区域，这表明皮层来源的神经干/祖细胞可能修复大脑皮层的缺血性病变。在目前的研究中，使用高度可重复的小鼠皮质梗死模型，我们发现巢蛋白阳性细胞存在于中风后的大脑皮层，但不存在于非缺血皮层。脑卒中后大脑皮质缺血核心细胞形成表达巢蛋白的神经球样细胞团；这些细胞具有自我更新的能力，并分化为具有电生理功能的神经元、星形胶质细胞和产生髓磷脂的少突胶质细胞。巢蛋白阳性细胞从中风影响的皮层迁移到梗死周围区域，并在体内分化为胶质细胞。虽然我们无法在体内检测到巢蛋白阳性细胞向神经元的分化，但我们目前的观察表明，具有成为神经元潜力的内源性神经干/祖细胞可以在中风后的大脑皮层内发育。
The CNS has the potential to marshal strong reparative mechanisms, including activation of endogenous neurogenesis, after a brain injury such as stroke. However, the response of neural stem/progenitor cells to stroke is poorly understood. Recently, neural stem/progenitor cells have been identified in the cerebral cortex, as well as previously recognized regions such as the subventricular or subgranular zones of the hippocampus, suggesting that a contribution of cortex-derived neural stem/progenitor cells may repair ischemic lesions of the cerebral cortex. In the present study, using a highly reproducible murine model of cortical infarction, we have found nestin-positive cells in the post-stroke cerebral cortex, but not in the non-ischemic cortex. Cells obtained from the ischemic core of the post-stroke cerebral cortex formed neurosphere-like cell clusters expressing nestin; such cells had the capacity for self-renewal and differentiated into electrophysiologically functional neurons, astrocytes and myelin-producing oligodendrocytes. Nestin-positive cells from the stroke-affected cortex migrated into the peri-infarct area and differentiated into glial cells in vivo. Although we could not detect differentiation of nestin-positive cells into neurons in vivo, our current observations indicate that endogenous neural stem/progenitors with the potential to become neurons can develop within post-stroke cerebral cortex.