Reactivation of larval keratin gene (krt62.L) in blastema epithelium during Xenopus froglet limb regeneration.
Reactivation of larval keratin gene (krt62.L) in blastema epithelium during Xenopus froglet limb regeneration.
复制标题
非洲爪蟾肢体再生过程中芽基上皮中幼虫角蛋白基因（krt62.L）的重新激活。
DOI：
10.1016/j.ydbio.2017.10.015
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发表时间：
2017
影响因子：
2.7
通讯作者：
Makanae A.
中科院分区：
文献类型：
--
作者：
Satoh A;Mitogawa K;Saito N;Suzuki M;Suzuki KT;Ochi H;Makanae A.
由于分子和形态的相似性，肢体再生被认为是肢体再开发的一种形式。形成再生胚本质上是在成体中形成一个发育中的肢芽。在一个成熟的身体中，这种发育过程的重新激活是值得研究的。非洲爪蟾有一个两阶段的生命周期，包括不同的幼虫和成虫阶段。这些不同的发育阶段对于研究变质后青蛙（小青蛙）的发育过程的重新激活是有用的。在这项研究中，我们重点研究了在爪蟾蛙肢体再生过程中一个幼虫基因（krt62.L）的重新表达。最近renamedkrt62。L，这个基因被称为幼虫角蛋白（xlk）基因，这是幼虫-蝌蚪阶段特有的。在小青蛙的肢体再生过程中，krt62。l在胚芽上皮基底层重新表达，在那里也可以观察到成人特异性角蛋白（Krt12.6.S）的表达。神经是两栖动物肢体再生的重要调控因子，也在胚基形成和维持中发挥重要作用。神经功能对krt62的影响。表达式可以在krt62的维护中看到。表达式，但不是在它的归纳。将表皮剥离的肢芽移植到小蛙胚中，可达到趾形成阶段。这表明krt62。l阳性的小青蛙胚母细胞上皮能够支持肢体发育过程。这些发现表明，在肢体再生过程中，发育过程在变形后的身体中被局部重新激活。
Limb regeneration is considered a form of limb redevelopment because of the molecular and morphological similarities. Forming a regeneration blastema is, in essence, creating a developing limb bud in an adult body. This reactivation of a developmental process in a mature body is worth studying.Xenopus laevishas a biphasic life cycle that involves distinct larval and adult stages. These distinct developmental stages are useful for investigating the reactivation of developmental processes in post-metamorphic frogs (froglets). In this study, we focused on the re-expression of a larval gene (krt62.L) duringXenopusfroglet limb regeneration. Recently renamedkrt62.L, this gene was known as thelarval keratin(xlk) gene, which is specific to larval-tadpole stages. During limb regeneration in a froglet,krt62.Lwas re-expressed in a basal layer of blastema epithelium, where adult-specific keratin (Krt12.6.S) expression was also observable. Nerves produce important regulatory factors for amphibian limb regeneration, and also play a role in blastema formation and maintenance. The effect of nerve function onkrt62.Lexpression could be seen in the maintenance ofkrt62.Lexpression, but not in its induction. When an epidermis-stripped limb bud was grafted in a froglet blastema, the grafted limb bud could reach the digit-forming stage. This suggests thatkrt62.L-positive froglet blastema epithelium is able to support the limb development process. These findings imply that the developmental process is locally reactivated in an postmetamorphic body during limb regeneration.