EFFECTS OF HIGH-CONCENTRATIONS OF GLUCOSE ON PTH SECRETION IN PARATHYROID CELLS
EFFECTS OF HIGH-CONCENTRATIONS OF GLUCOSE ON PTH SECRETION IN PARATHYROID CELLS
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DOI：
10.1038/ki.1990.144
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发表时间：
1990-06-01
影响因子：
19.6
通讯作者：
SLATOPOLSKY, E
中科院分区：
文献类型：
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作者：
SUGIMOTO, T;RITTER, C;SLATOPOLSKY, E
我们研究了高浓度葡萄糖对培养的牛甲状旁腺细胞分泌PTH的影响。增加葡萄糖浓度可抑制PTH的分泌。在用低至15 mM葡萄糖孵育48小时内发现PTH分泌的显著抑制。向培养基中加入氯化胆碱并不抑制PTH分泌，尽管渗透压与含有50 mM葡萄糖的培养基相同。当先前暴露于50 mM葡萄糖的细胞在含有5 mM葡萄糖的培养基中再孵育48小时时，观察到PTH分泌完全恢复。在暴露于50 mM葡萄糖的细胞中，PTH分泌对10-6 M异丙肾上腺素的响应幅度变钝。酸性尿素凝胶电泳显示，高浓度葡萄糖暴露的细胞分泌的完整PTH和片段的模式与对照细胞相似。从培养基中去除胰岛素导致PTH分泌的抑制，类似于高浓度葡萄糖下观察到的变化。高浓度葡萄糖和缺乏胰岛素的抑制作用是相加的。然而，我们不能从目前的研究中排除缺乏胰岛素对PTH分泌的抑制作用是否继发于维持甲状旁腺细胞可能需要胰岛素的事实。目前的研究表明，葡萄糖直接调节甲状旁腺原代细胞培养的PTH分泌。
We examined the effects of high concentrations of glucose on PTH secretion from cultured bovine parathyroid cells. Increasing medium concentration of glucose caused suppression of PTH secretion. A significant suppression of PTH secretion was found within 48 hours of incubation with as little as 15 mM glucose. The addition of choline chloride to the medium did not suppress PTH secretion, although the osmolality was the same as that of the medium containing 50 mM glucose. When cells previously exposed to 50 mM glucose were reincubated in the medium containing 5 mM glucose for another 48 hours, a complete recovery of PTH secretion was observed. In the cells exposed to 50 mM glucose, the magnitude of the response of PTH secretion to 10-6 M isoproterenol was blunted. Acid-urea gel electrophoresis revealed that the pattern of intact PTH and fragments secreted from cells exposed to high concentration of glucose was similar to that from control cells. Removal of insulin from the medium resulted in a suppression of PTH secretion similar to changes observed with high concentrations of glucose. The suppressive effects of high concentrations of glucose and lack of insulin were additive. However, we cannot exclude from the present studies whether the suppressive effects of the lack of insulin on PTH secretion was secondary to the fact that insulin may be required for the maintenance of parathyroid cells. The present studies demonstrate that glucose directly modulates PTH secretion in primary parathyroid cell culture.