Visualization and exact molecular weight determination of a Rhizobium meliloti megaplasmid.
Visualization and exact molecular weight determination of a Rhizobium meliloti megaplasmid.
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苜蓿根瘤菌大质粒的可视化和精确分子量测定。
DOI：
10.1016/0022-2836(84)90475-3
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发表时间：
1984
影响因子：
5.6
通讯作者：
H. Burkardt
中科院分区：
文献类型：
--
作者：
B. Burkardt;H. Burkardt
采用温和裂解和蔗糖梯度离心法分离苜蓿根瘤菌MVII/1细胞的全DNA。蔗糖梯度的馏分进行了研究，通过电子显微镜。我们发现了超螺旋和松弛形式的完整巨质粒和总染色体。可以测量巨质粒的长度，这允许精确测定分子量。我们发现长度为0.48 ± 0.019 mm，相当于分子量约为1000 × 106。
The entire DNA ofRhizobium melilotiMVII/1 cells was isolated preparatively by gentle lysis and sucrose gradient centrifugation. Fractions of the sucrose gradient were investigated by electron microscopy. We found intact megaplasmids in supercoiled and relaxed form and total chromosomes. The length of the megaplasmid could be measured, which allowed an exact determination of the molecular weight. We found a length of 0.48 ± 0.019 mm equivalent to a molecular weight of about 1000 × 106.