Development of Heat Hyperalgesia and Changes of TRPV1 and NGF Expression in Rat Dorsal Root Ganglion Following Joint Immobilization
Development of Heat Hyperalgesia and Changes of TRPV1 and NGF Expression in Rat Dorsal Root Ganglion Following Joint Immobilization
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DOI：
10.33549/physiolres.932280
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发表时间：
2013-01-01
影响因子：
2.1
通讯作者：
Ushida, T.
中科院分区：
文献类型：
--
作者：
Nishigami, T.;Osako, Y.;Ushida, T.
本研究的目的是研究长期肢体制动条件下背根神经节（DRG）对热刺激的阈值以及瞬时受体电位香草醛1（TRPV 1）和神经生长因子（NGF）的表达是否发生变化。将塑料管型从前臂到前爪包裹在右侧肢体周围，以保持腕关节屈曲90 °，持续5周。取石膏后6 h，用足底实验检测热痛敏。取管型后24 h取背根神经节（DRG），用4%多聚甲醛灌流.对于TRPV 1-IR和NGF-IR神经元谱的大小分布，在固定（同侧）和对侧的每侧测量每只动物超过1000个DRG神经元的DRG面积测量值。同侧回缩潜伏期明显短于对侧。长时间铸型引起热痛敏，固定侧背根神经节TRPV 1-IR和NGF-IR表达上调并发生表型改变。这些DRG改变可能涉及长期肢体制动后的热痛觉过敏。
The aim of this study was to examine whether threshold to heat stimuli, and expression of transient receptor potential vanilloid1 (TRPV1) and nerve growth factor (NGF) in dorsal root ganglion (DRG) altered under conditions of long-term limb immobilization. A plastic cast was wrapped around the right limb from the forearm to the forepaw to keep wrist joint at 90 of flexion for 5 weeks. Heat hyperalgesia was tested using the plantar test at 6 h after removing cast. The rats were perfused transcardially with 4 % paraformaldehyde and DRGs were excised at 24 h after removing cast. For size distributions of the TRPV1-IR and NGF-IR neuronal profile, the DRG area measurements over 1000 DRG neurons per animal were measured in each side, on both the immobilized (ipsilateral) and contralateral sides. Ipsilateral withdrawal latency was significantly shorter than contralateral sides. Ipsilateral percentage of immunoreactive neurons in the total DRG neurons was significantly higher than contralateral sides in TRPV1-IR and NGF-IR. Long-term casting induced heat hyperalgesia, and up-regulation and phenotypic change of TRPV1-IR and NGF-IR in DRGs on the immobilized side. These DRG alterations may involve heat hyperalgesia after long-term limb immobilization.