Activating an anterior nucleus gigantocellularis subpopulation triggers emergence from pharmacologically-induced coma in rodents
Activating an anterior nucleus gigantocellularis subpopulation triggers emergence from pharmacologically-induced coma in rodents
复制标题
DOI：
10.1038/s41467-019-10797-7
复制
发表时间：
2019-07-01
影响因子：
16.6
通讯作者：
Pfaff, D. W.
中科院分区：
文献类型：
--
作者：
Gao, S.;Proekt, A.;Pfaff, D. W.
网状激活系统（RAS）内的多个区域可以加速从睡眠或轻度麻醉中醒来。然而，个别部位的刺激显示出从大脑活动的深度全局抑制（例如昏迷）中的有限恢复。在这里，我们确定了RAS神经元的一个子集内的前部分的神经细胞核（aNGC）能够产生高度的觉醒代表广泛的高频皮层再激活与有组织的运动和行为反应的环境从两个不同的模型的深度药理学诱导的昏迷（PIC）：异氟烷（1.25%-1.5%）和诱导的低血糖昏迷。激活aNGC神经元通过募集胆碱能、去甲肾上腺素能和多巴胺能唤醒通路触发觉醒。总之，我们确定了一个进化上保守的RAS神经元群体，通过协调激活多个唤醒促进回路，广泛恢复啮齿动物的大脑皮层激活和运动行为。
Multiple areas within the reticular activating system (RAS) can hasten awakening from sleep or light planes of anesthesia. However, stimulation in individual sites has shown limited recovery from deep global suppression of brain activity, such as coma. Here we identify a subset of RAS neurons within the anterior portion of nucleus gigantocellularis (aNGC) capable of producing a high degree of awakening represented by a broad high frequency cortical reactivation associated with organized movements and behavioral reactivity to the environment from two different models of deep pharmacologically-induced coma (PIC): isoflurane (1.25%-1.5%) and induced hypoglycemic coma. Activating aNGC neurons triggered awakening by recruiting cholinergic, noradrenergic, and glutamatergic arousal pathways. In summary, we identify an evolutionarily conserved population of RAS neurons, which broadly restore cerebral cortical activation and motor behavior in rodents through the coordinated activation of multiple arousal-promoting circuits.