The lacrimal gland expresses nuclear retinoid X receptors.
The lacrimal gland expresses nuclear retinoid X receptors.
复制标题
泪腺表达核视黄醇 X 受体。
DOI：
10.1076/ceyr.16.5.511.7045
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发表时间：
1997
影响因子：
2
通讯作者：
Viel,MD
中科院分区：
文献类型：
--
作者：
Ubels,JL;Sulahian,TH;Viel,MD
PURPOSE泪腺表达核维甲酸受体。这表明维甲酸参与了泪腺基因表达的控制。维甲酸X受体(RXRs)与维甲酸受体形成异二聚体，是维甲酸受体激活所必需的。本研究的目的是鉴定泪腺中的维甲酸X受体。方法从大鼠、兔和人的泪腺和培养的大鼠泪腺细胞中提取RNA。用RXRA、RXR？和RXR？的cDNA探针进行Northern印迹分析。用鼠单抗免疫印迹法检测大鼠和兔泪腺核蛋白提取物中的RXR？结果在所有物种的泪腺和培养的大鼠泪腺细胞中均存在RXR？RXR？仅在兔泪腺组织中检测到1.9kb的mRNA。RXR？在大鼠和兔泪腺中表达为50 kDa的蛋白质。结论本研究证实了RXR在泪腺中的存在，从而支持视黄醇及其核受体在泪腺基因转录调控中的作用。
PURPOSEThe lacrimal gland expresses nuclear retinoic acid receptors. This suggests that retinoids are involved in control of gene expression in the lacrimal gland. Retinoid X receptors (RXRs) form heterodimers with and are required for activation of retinoic acid receptors. The purpose of this study was to identify retinoid X receptors in the lacrimal gland.METHODSTotal RNA was purified from rat, rabbit and human lacrimal glands and from cultured rat lacrimal cells. RNA was analyzed by northern blotting using cDNA probes for RXRa, RXRß and RXR?. Nuclear protein extracts from rat and rabbit lacrimal glands were probed for RXRß by immunoblotting, using a mouse monoclonal antibody.RESULTSRXRa mRNA transcripts (5 kb) and RXRß mRNA transcripts (3.3 kb) are present in the lacrimal glands of all species studied and in cultured rat lacrimal cells. RXR? mRNA (1.9 kb) was detected only in the rabbit lacrimal gland. RXRß is expressed as a 50 kDa protein in rat and rabbit lacrimal glands.CONCLUSIONSThis study confirms the presence of RXRs in the lacrimal gland, thereby supporting a role for retinoids and their nuclear receptors in the control of gene transcription in the lacrimal gland.