Changes in distribution of serotonin induced by spinal injury in larval lampreys: Evidence from immunohistochemistry and HPLC
Changes in distribution of serotonin induced by spinal injury in larval lampreys: Evidence from immunohistochemistry and HPLC
复制标题
DOI：
10.1089/neu.2005.22.172
复制
发表时间：
2005-01-01
影响因子：
4.2
通讯作者：
Chakrabarti, L
中科院分区：
文献类型：
--
作者：
Cohen, AH;Abdelnabi, M;Chakrabarti, L
已知七鳃鳗幼体在脊髓损伤后能成功恢复正常行为。最近，我们发现温度可以影响功能恢复，尽管寒冷是动物的正常温度，但较低的温度更有可能产生行为异常。在这里，我们分析与温度效应相关的差异。我们研究了在考虑损伤时间、恢复过程中的温度和纤维位置的情况下，脊髓中段损伤后沿着脊髓的多巴胺能表达。我们还研究了再生和运动之间的关系，只要在无限制的游泳过程中的肌肉电位的模式是正常或异常。在脊髓损伤后26周，在所有组的动物中，免疫组化和HPLC的5-羟色胺和5-羟色胺的表达以上和以下的病变可以显着改变，在所有区域来源的5-羟色胺独立的温度动物从他们的损伤中恢复。来自暖箱的动物在病变部位尾部立即恢复5-羟色胺表达，距离病变稍远;来自冷室水族箱的动物在病变尾部的表达恢复明显较少，并且没有更远。5-羟色胺的分布和恢复之间没有明显的关系。这些变化表明发生了一些椎管内重组。我们提出了观察结果和功能恢复之间的关系，但它仍然是不确定的。一些动物恢复正常功能的事实表明，可塑性一定发生在成功恢复正常功能的动物身上。因此，七鳃鳗可以作为一个模型系统来研究允许或阻止功能恢复的适应性变化。
Larval lampreys are known to successfully recover normal behavior following spinal cord injury. More recently, we showed temperature can influence functional recovery, with colder temperatures more likely producing behavioral abnormality despite the cold being the animals' normal temperature. Here we analyze the differences associated with temperature effects. We examine serotonergic expression along the spinal cord following midbody lesions considering time from injury, temperature during recovery and fiber location. We also examine the relationship between regeneration and locomotion, insofar as the pattern of muscle potentials during unrestrained swimming is normal or abnormal. At 26 weeks after spinal cord injury in all groups of animals, immunohistochemistry and HPLC for serotonin and serotonin expression above and below the lesion can be significantly changed in all regional sources of serotonin independent of the temperature animals recover from their injuries. Animals from warmer tanks recover serotonin expression in the segment immediately caudal to the lesion site with little further away from the lesion; animals from the cold room aquaria have significantly less recovery of expression caudal to the lesion and none further away. There was no apparent relationship between the distribution of serotonin and recovery. The changes suggest that some intraspinal reorganization has occurred. We propose a relationship between the observed results and functional recovery, but it remains conjectural. The fact that some animals recover normal function suggests plasticity must occur in animals successful in recovering normal function. Thus, the lamprey can be used as a model system to study the adaptive changes that permit or prevent functional recovery.