A Novel Cross-linked Poly(vinyl alcohol) (PVA) for Vascular Grafts
A Novel Cross-linked Poly(vinyl alcohol) (PVA) for Vascular Grafts
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DOI：
10.1002/adfm.200701261
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发表时间：
2008-10-09
影响因子：
19
通讯作者：
Letourneur, Didier
中科院分区：
文献类型：
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作者：
Chaouat, Marc;Le Visage, Catherine;Letourneur, Didier
聚乙烯醇（PVA）是一种水溶性合成聚合物，具有优异的成膜性、乳化性和粘合性。本研究的目的是设计一种简单的工艺，使聚乙烯醇与三偏磷酸钠交联，形成适用于生物医学应用的膜装置。该方法不需要有机溶剂，也不需要熔融过程以获得具有高机械强度的膜。从PVA膜制造小直径管是容易的，具有围绕特氟隆棒的单个缠绕步骤。动态力学分析表明，在去除所施加的应力后，具有2 × 10（5）kPa杨氏模量的PVA膜恢复到其原始尺寸和形状。PVA管的壁厚为344 ± 13 μ m（n = 12），接近于人体动脉的壁厚（350-710 μ m）。PVA管的缝线保持力极佳（140 +/- 11 g），接近人体血管的缝线保持力。发现PVA管的爆破压力为507 +/- 25 mm Hg，比人类健康收缩动脉压高三倍多。在动脉压下，即使在针穿刺后也没有泄漏，与临床血管膨体聚四氟乙烯假体相反。最后，使用直径为2 mm的PVA管来代替大鼠肾下主动脉的一段。至少一周内，即使没有抗凝剂或抗血小板治疗，也没有发现机械或血栓并发症。移植物通畅性也可通过非侵入性成像技术证明。总之，这种新的交联方法赋予聚（乙烯醇）特定的机械性能，如顺应性，弹性和耐机械应力，与循环血流相容。
Poly(vinyl alcohol) (PVA) is a water-soluble synthetic polymer with excellent film-forming, emulsifying, and adhesive properties. The aim of this study is to design a simple process for PVA cross-linking with sodium trimetaphosphate to form membrane devices suitable for biomedical applications. This procedure requires no organic solvent, nor melting process to obtain films with high mechanical strength. Fabrication of a small diameter tube from a PVA film is easy with a single wrapping step around a Teflon rod. Dynamic mechanical analysis demonstrated that, upon removal of the applied stress, the PVA film with a Young's modulus of 2 x 10(5) kPa returns to its original size and shape. The wall thickness of PVA tubes is 344 +/- 13 mu m (n = 12), which is close to the wall thickness of a human artery (350-710 mu m). Suture retention of a PVA tube is excellent (140 +/- 11 g), close to that of human vessels. The burst pressure of PVA tubes is found to be 507 +/- 25 mm Hg, more than three times higher than the human healthy systolic arterial pressure. Under arterial pressure, there was no leakage even after needle puncture, contrary to clinical vascular expanded polytetrafluoroethylene prostheses. Finally, PVA tubes of 2 mm in diameter are used to replace a segment of an infrarenal aorta in rats. For at least one week, no mechanical nor thrombotic complications are noticed even in the absence of anticoagulant or antiplatelet treatment. Graft patency is also evidenced with non-invasive imaging techniques. As a conclusion, this novel cross-linking method confers to poly(vinyl alcohol) particular mechanical properties such as compliance, elasticity and resistance to mechanical stress, compatible with the circulatory blood flow.