MYB98 is required for pollen tube guidance and synergid cell differentiation in Arabidopsis
MYB98 is required for pollen tube guidance and synergid cell differentiation in Arabidopsis
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DOI：
10.1105/tpc.105.034603
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发表时间：
2005-11-01
期刊：
影响因子：
11.6
通讯作者：
Drews, GN
中科院分区：
文献类型：
--
作者：
Kasahara, RD;Portereiko, MF;Drews, GN
雌配子体的助细胞在被子植物受精过程的许多步骤中发挥作用，包括指导雌配子体的花粉管生长。然而，助细胞在雌配子体发育过程中变得特定并形成其独特特征的机制尚不清楚。我们在筛选雌性配子体中表达的拟南芥基因中鉴定了MYB 98。MYB 98是R2 R3-MYB基因家族的成员，该家族的成员可能编码转录因子。在胚珠中，MYB 98只在助细胞中表达，该基因的突变特异性地影响雌性配子体。myb 98雌配子体在助细胞的两个独特特征，即花粉管引导和丝状器方面受到影响，但在其他方面是正常的。MYB 98也在毛状体和胚乳中表达。纯合myb 98突变体没有表现出孢子体缺陷，包括毛状体和胚乳缺陷。总之，这些数据表明，MYB 98控制雌配子体发育过程中助细胞内特定特征的发育。
The synergid cells of the female gametophyte play a role in many steps of the angiosperm fertilization process, including guidance of pollen tube growth to the female gametophyte. However, the mechanisms by which the synergid cells become specified and develop their unique features during female gametophyte development are not understood. We identified MYB98 in a screen for Arabidopsis thaliana genes expressed in the female gametophyte. MYB98 is a member of the R2R3-MYB gene family, the members of which likely encode transcription factors. In the context of the ovule, MYB98 is expressed exclusively in the synergid cells, and mutations in this gene affect the female gametophyte specifically. myb98 female gametophytes are affected in two unique features of the synergid cell, pollen tube guidance and the filiform apparatus, but are otherwise normal. MYB98 also is expressed in trichomes and endosperm. Homozygous myb98 mutants exhibit no sporophytic defects, including trichome and endosperm defects. Together, these data suggest that MYB98 controls the development of specific features within the synergid cell during female gametophyte development.