Identification of Granulicatella elegans from a case of infective endocarditis, based on the phenotypic characteristics and 16S rRNA gene sequence
Identification of Granulicatella elegans from a case of infective endocarditis, based on the phenotypic characteristics and 16S rRNA gene sequence
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DOI：
10.2459/jcm.0b013e328356a471
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发表时间：
2015-01
影响因子：
3
通讯作者：
Ming-Yi Wang;Jing Lin;Sheng-bo Sun;Xiao-yan Yuan
中科院分区：
文献类型：
--
作者：
Ming-Yi Wang;Jing Lin;Sheng-bo Sun;Xiao-yan Yuan
感染性心内膜炎是一种罕见的危及生命的心脏病，它是常规诊断的基础上存在的赘生物超声心动图和阳性血培养。然而，一些罕见的和难养的微生物也可能是感染性心内膜炎的病原体，包括营养不良菌、HACEK组（嗜血杆菌属，伴放线放线杆菌，人心杆菌，腐蚀艾肯氏菌和金氏氏菌），布鲁氏菌属，巴尔通体属，军团菌属和支原体属（Mycoplasma spp.）其中，感染性心内膜炎的主要病原体是营养变异链球菌（NVS），其需要特定的培养基和条件，如富含L-半胱氨酸的培养基。优雅颗粒菌是这组细菌中最挑剔的成员之一，最初于1961年被描述为NVS家族的成员。此后，柯林斯和劳森提出G. elegans被重新分类为一个新属，Granulicatella。G.在一名感染性心内膜炎患者的血液样本中发现了秀丽线虫，它也被认为是所谓的“培养阴性心内膜炎”的重要病原体之一。同时，该物种的一些菌株不能很容易地确定，因为它们的一些表型特征与文献中公布的物种描述不相关。分子生物学方法，如PCR扩增和DNA测序，在菌株鉴定中是有价值的。在这项研究中，我们能够识别G。通过表型特征和16 S rRNA基因序列分析，从血培养阳性的感染性心内膜炎患者中分离到一株秀丽线虫。
To the Editor Infective endocarditis is an uncommon life-threatening heart disease, and it is routinely diagnosed based on the presence of vegetations on echocardiography and positive blood cultures. However, some rare and fastidious microorganisms may also be the aetiologic agents of infective endocarditis, including Abiotrophia, the HACEK group (Haemophilus spp., Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, Eikenella corrodens and Kingella kingae), Brucella spp., Bartonella spp., Legionella spp. and Mycoplasma spp. Among them, some predominant pathogens of infective endocarditis are nutritionally variant streptococci (NVS), which require specific media and conditions such as L-cysteine-enriched medium. Granulicatella elegans is one of the most fastidious members among this group of bacteria, which was originally described as a member of a family of NVS in 1961. Since then, Collins and Lawson have proposed that G. elegans be reclassified with other species in a new genus, Granulicatella. G. elegans were found in a blood specimen from a patient suffering from infective endocarditis, and it was also regarded as one of the important pathogens in so-called ‘culture-negative endocarditis’. Meanwhile, some strains of the species could not be determined easily because some of their phenotypic characteristics did not correlate with the species descriptions published in the literature. Molecular methods, such as PCR amplification and DNA sequencing, are valuable in strain identification. In the study we were able to identify G. elegans from a patient with blood culture-positive infective endocarditis by phenotypic characteristics and 16S rRNA gene sequence.